使用扫描离子导电显微镜探索中性粒细胞外细胞迁移的过程
Svetlana N Pleskova1,2, Nikolay A Bezrukov1, Ekaterina N Gorshkova1
1Research Laboratory of Scanning Probe Microscopy, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, Russia.
Cells
|July 14, 2023
概括
在败血症期间的中性粒细胞迁移会导致内皮细胞损伤. 扫描离子导电显微镜揭示了中性粒细胞形成细胞外陷和群,增加内皮细胞之间的间隙,导致细胞死亡.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 显微镜的使用方法
背景情况:
- 在炎症和传染性疾病中,中性粒细胞的超内皮细胞迁移至关重要.
- 败血症涉及细菌感染和全身炎症,影响中性粒细胞的行为和内皮完整性.
研究的目的:
- 在实验性败血症中研究中性粒细胞和内皮细胞在超内皮细胞迁移期间的动态变化.
- 用扫描离子导电显微镜来描述内皮细胞的形态变化.
主要方法:
- 由黄金葡萄球菌 (Staphylococcus aureus) 引起的实验性败血症模型.
- 扫描离子导电显微镜 (SICM) 用于细胞形态和动态的高分辨率成像.
- 对中性粒细胞行为 (爬行,蜂群,NETs形成) 和内皮细胞变化 (粘附,膜完整性,刚性) 的分析.
主要成果:
- 中性粒细胞迁移涉及爬行,蜂群和中性粒细胞外细胞陷 (NETs) 的形成.
- 中性粒细胞的蜂群与内皮细胞之间的细胞间空间增加有关.
- 内皮细胞的粘附性和膜光滑性降低,但矛盾的是,硬度没有改变. 渐进的损伤包括穿孔,应力纤维的形成,以及最终的细胞死亡.
结论:
- 在败血症中,中性粒细胞的跨内皮细胞迁移会导致内皮细胞结构和功能的显著,渐进的改变.
- 这项研究强调了严重感染期间中性粒细胞和内皮之间的复杂相互作用,导致屏障功能障碍和细胞死亡.
关键词:
网络 网络 网络 网络黄金葡萄球菌黄金葡萄球菌变化 改变 改变细胞的刚性 细胞的刚性内皮细胞是内皮细胞.这是一种炎症炎症炎症炎症.作为一个中性恋者.扫描离子导电显微镜扫描.已经有了 септикопиемия.超内皮细胞迁移的跨内皮细胞迁移.更多相关视频
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