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相关概念视频

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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相关实验视频

Updated: Jul 23, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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在雅克卵细胞成熟期间蛋白质组的动态变化使用iTRAQ技术分析.

Xin Ma1,2, Meng Wang1,2, Jinglei Wang1,2

  • 1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.

Animals : an open access journal from MDPI
|July 14, 2023
PubMed
概括
此摘要是机器生成的。

雅克卵细胞成熟涉及动态的蛋白质组变化,在不同阶段具有不同的代谢和遗传活动. 了解这些蛋白转移有助于分析雅克卵细胞发育的分子调节.

关键词:
不同的蛋白质组学.这就是iTRAQQ.卵子细胞 卵子细胞没有了,没有了,没有了.

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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
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Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
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相关实验视频

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科学领域:

  • 生殖生物学 生殖生物学
  • 蛋白质组学是指蛋白质组学.
  • 分子生物学分子生物学

背景情况:

  • 雅克卵细胞的成熟对于繁殖成功至关重要.
  • 了解控制卵细胞成熟的分子机制至关重要.

研究的目的:

  • 为了研究雅克卵细胞在体外成熟期间的动态蛋白质变化.
  • 确定不同阶段 (GV,MI,MII) 参与雅克卵细胞发育的关键蛋白质和途径.

主要方法:

  • 雅克卵细胞的体外成熟.
  • 使用 iTRAQ 技术进行差异蛋白质组学测序.
  • 生物信息分析包括基因本体学 (GO),KOG和KEGG通路分析.

主要成果:

  • 确定了与代谢过程和分子结合相关的差异性蛋白质.
  • 观察到从GV到MI阶段的能量代谢增加,以及从MI到MII阶段的遗传物质准备.
  • 丰富的途径包括PPAR,Hippo信号传递,ECM-受体相互作用 (GV-MI) 和PI3K-Akt,TGF-β,细胞体 (MI-MII).

结论:

  • 在雅克卵细胞成熟期间发生了显著的蛋白质和功能转变.
  • 特定的途径被动态调节,影响卵细胞的发育.
  • 这项研究提供了关于雅克卵细胞成熟的分子调节机制的见解.