日本青中MHC超型的保存进化表明选择Bd抗性
Quintin Lau1, Takeshi Igawa2, Tiffany A Kosch3
1Research Center for Integrative Evolutionary Science, Sokendai (The Graduate University for Advanced Studies), Hayama 240-0115, Japan.
东亚青拥有保存的MHC-IIb等位基因,这表明它们与菌Batrachochytrium dendrobatidis (Bd) 的共同进化适应. 这种MHC变异可能会对Bd产生耐药性,Bd是源自该地区的两动物威胁.
科学领域:
- 两动物免疫学 两动物免疫学
- 进化遗传学的进化遗传学
- 病原体的耐药性 病原体的耐药性
背景情况:
- 菌菌 Batrachochytrium dendrobatidis (Bd) 在全球对两动物构成严重威胁.
- 尽管Bd的影响很大,但东亚青的疾病爆发报告有限.
- 主要基因相容性复合体 (MHC) 的遗传变异与Bd耐药性有关.
研究的目的:
- 为了研究日本青中MHC类I和IIb基因序列.
- 为了比较东亚青的MHC变异与世界各地的物种关于Bd易感性的变化.
- 评估MHC变异对Bd-结合的功能影响.
主要方法:
- 分析了11种日本青的转录数据.
- 通过分子克隆将MHC类I和IIb序列分离并验证.
- 超类型分析根据结位属性对MHC等位基进行了分组.
- NetMHCIIpan-4.0软件预测了潜在的Bd-结合亲缘关系.
主要成果:
- 所有被检查的东亚青在ST-1超型中至少具有一个MHC-IIb等位基因.
- 在不同的日本青物种中,MHC-II结位的功能性质得到了保留.
- MHC-IIb超型ST-1和ST-2对Bd和其他微生物具有更高的预测结能力.
结论:
- 东亚青中保存的MHC-IIb等位基因表明它们适应了共同的选择性压力.
- 东亚Bd的起源可能推动了当地青种群的MHC进化.
- MHC-IIb变异可能在两动物对Bd的耐药性中起着至关重要的作用.
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