从自然感染的哺乳动物中分离出来的Leishmania寄生虫的特征
Aroia Burguete-Mikeo1,2, Celia Fernández-Rubio1,2, José Peña-Guerrero1,2
1ISTUN Instituto de Salud Tropical, Department of Microbiology and Parasitology, University of Navarra, c/Irunlarrea 1, E-31008 Pamplona, Spain.
Animals : an open access journal from MDPI
|July 14, 2023
概括
来自狗的新莱什马尼亚婴儿菌株 (NAV和TDL) 显示感染能力和药物敏感性增加. 这些发现对于了解地中海地区的莱什曼病至关重要.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 由于气候变化和移民,莱什曼病在欧洲正在扩散.
- 婴儿莱什曼尼亚是主要原因,但新型菌株需要进一步表征.
- 了解寄生虫生物学是打击莱什曼病传播的关键.
研究的目的:
- 来自自然感染的狗的新型Leishmania婴儿分离物 (NAV和TDL) 的特征.
- 为了比较NAV和TDL菌株与参考菌株 (BCN 150) 在生长,药物敏感性和基因表达方面.
- 为了评估细胞内阿马斯蒂哥特的感染性和药物敏感性.
主要方法:
- 从受感染的狗中分离和鉴定Leishmania婴儿菌株的特征.
- 对前列腺细胞生长速度和药物敏感性的比较分析 (安福特素B,米尔特福辛).
- 定量基因表达分析 (CYCA,CYC6,APG9) 和体外感染分析.
主要成果:
- 与BCN 150相比,NAV和TDL分离物体的生长速度较慢,对安福特里辛B和米尔特福辛的敏感性增加.
- 在NAV和TDL中观察到CYCA的下调和CYC6和APG9基因的上调.
- 这两种隔离物体在细胞内巨阶段表现出更高的感染能力和更高的对米尔特福辛的敏感性.
结论:
- TDL和NAV代表具有独特生物特征的新型Leishmania分离物.
- 这些菌株为地中海特有地区的寄生虫生物学体外和体内研究提供了有价值的模型.
- 对这些分离物的进一步研究可以增强我们对莱什曼病原体和治疗耐药性的理解.
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