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Updated: Jul 23, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

循环RNA作为动脉样硬化治疗点:我们在循环中运行吗?

Jeffrey Triska¹, Christo Mathew¹, Yang Zhao²

¹Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.

Journal of clinical medicine

|July 14, 2023

概括

针对诸如动脉样硬化等心血管疾病的循环RNA (circRNA) 和微RNA (miRNA) 的治疗向显示出潜力,但具有重大风险. 不能预测的脱效应使circRNA和miRNA的操纵变得复杂和危险.

科学领域:

心血管研究研究心血管研究
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

心血管疾病,包括动脉样硬化,涉及复杂的遗传因素.
非编码RNAs,如循环RNAs (circRNAs) 和微RNAs (miRNAs),正在研究它们在心血管疾病中的诊断和治疗潜力.
评估基于circRNA的疗法的风险,益处和长期非目标效应至关重要.

研究的目的:

从2016-2022年审查和分析关于circRNAs和动脉样硬化的现有文献.
在动脉样硬化背景下研究circRNAs的机制,表达模式和影响.
在动脉样硬化模型中识别和评估关于circRNA和miRNA操纵的发现的一致性.

主要方法:

在PubMed数据库中系统搜索关于circRNA和动脉样硬化 (2016-2022) 的出版物.
审查研究设计,包括动脉样硬化诱导方法及其对circRNA表达的影响.
分析研究的机制,包括miRNA相互作用,基因点,以及对细胞力学和炎症标记物的影响.

主要成果:

在研究中观察到许多不同的,相反的和矛盾的发现.
在动脉样硬化环境中,特定circRNAs的表达水平显著变化.
循环RNA或miRNA沉默对动脉样硬化进展产生了不一致的影响,显著的非目标,细胞特异和疾病特异性影响.

结论:

针对心血管疾病的circRNAs和miRNAs的治疗向是复杂的,原因是不一致的发现和可变的表达.
存在重大潜在的有害和不可预测的非目标效应.
目前的理解表明,在动脉样硬化中对circRNA或miRNA分子的治疗操纵可能是复杂和危险的.

关键词:

动脉样硬化动脉样硬化心血管疾病心血管疾病圆形的RNARNA是一个圆形的RNA.微型RNA微型RNA是什么潜在的治疗治疗方法.

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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