基于LC-MS/MS的血清代谢和转录组分析,用于增强脏干的机制
Yidan Wang1,2, Yifan Luo1,2, Shu Yang1,2
1Department of Pharmacy, The First Hospital of China Medical University, Shenyang 110001, China.
International journal of molecular sciences
|July 14, 2023
概括
在重症患者中,增加的脏清除 (ARC) 涉及改变的纯氨酸,氨酸和阿拉基酸代谢. 这项研究使用了转录学和代谢学来识别关键的基因和代谢物,这些基因和代谢物参与了ARC病变的产生.
科学领域:
- 关键护理医学 关键护理医学
- 药物基因组学 药物基因组学
- 脏生理学 脏生理学
背景情况:
- 提升脏清除 (ARC) 的特点是,在重症患者中,溶液的脏清除增加.
- 了解ARC背后的分子机制对于优化药物剂量和患者结果至关重要.
研究的目的:
- 在转录和代谢水平上调查增强脏清除 (ARC) 的病原性.
- 通过综合转录和代谢分析,识别与ARC相关的关键基因和代谢物.
主要方法:
- 来自5个基因表达大数据集的534个样本的转录组分析,以确定834个差异基因.
- 在102个样本上使用超高性能液体染色学-四极点飞行时间质谱学进行代谢分析,以确定45种差异性代谢物.
- 结合转录组和代谢组分析以阐明ARC的病原性.
主要成果:
- 在ARC患者中,确定了 purin代谢, arginine生物合成和 arachidonic 酸代谢的显著变化.
- 突出了LTB4R,ARG1,ALOX5,阿尔金因和前列腺素E2在调节脏血液输液中的潜在作用.
- 建议CA4,PFKFB2,PFKFB3,PRKACB,NMDAR,谷氨酸和cAMP参与调节毛细血管壁的透性.
结论:
- ARC的发病与脏中复杂的代谢和转录变化有关.
- 确定了特定的代谢途径和分子参与者,可以作为管理ARC的治疗点.
相关概念视频
01:06Renal Drug Clearance: Overview
255
Renal clearance is a crucial parameter in pharmacokinetics that quantifies the rate at which the kidneys excrete a drug. It represents a constant fraction of the central volume of distribution containing the drug that the kidney eliminates per unit of time.
Renal clearance can be calculated using different methods. One approach is to divide the urinary drug excretion rate by the plasma drug concentration. This method directly measures renal clearance, indicating the kidneys' efficiency in...
Renal clearance can be calculated using different methods. One approach is to divide the urinary drug excretion rate by the plasma drug concentration. This method directly measures renal clearance, indicating the kidneys' efficiency in...
255
01:08Renal Drug Clearance: Comparison Between Renal Excretion Methods
181
Renal clearance is a critical parameter encompassing kidney filtration, secretion, and reabsorption processes. It is calculated using a specific equation to determine the rate at which the kidneys clear a drug.
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
181
01:31Factors Affecting Renal Clearance: Drug's Physicochemical Properties and Plasma Levels
267
Renal clearance of a drug is influenced by various factors, including its physicochemical properties and plasma levels. These factors play a significant role in determining how efficiently the kidneys eliminate a drug.
One important factor is the drug's molecular size. The kidneys readily excrete smaller molecules below 300 Daltons (Da). On the other hand, molecules weighing between 300 and 500 Da are excreted through both urine and bile. Larger molecules above 500 Da tend to be excreted...
One important factor is the drug's molecular size. The kidneys readily excrete smaller molecules below 300 Daltons (Da). On the other hand, molecules weighing between 300 and 500 Da are excreted through both urine and bile. Larger molecules above 500 Da tend to be excreted...
267
01:24Serum Studies: Renal Function Tests
23
Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
23
01:17Factors Affecting Renal Clearance: Renal Impairment
123
Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
123
01:23Renal Clearance
1.1K
The glomerular filtration rate (GFR) is a critical marker of kidney function, reflecting the efficiency of filtration by the glomeruli. Renal clearance of specific substances, such as inulin or creatinine, is commonly used to measure GFR.
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
1.1K