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在B细胞中增加的奥托费林表达与未经治疗的青少年皮肤肌炎的肌肉软弱有关:试点研究
Ameera Bukhari1, Amer Khojah2,3, Wilfredo Marin4
1College of Science, Taif University, Taif 21944, Saudi Arabia.
International journal of molecular sciences
|July 14, 2023
概括
青少年皮肤肌炎 (JDM) 患者的奥托弗林mRNA表达升高,与疾病活性和肌肉疲弱相关. B细胞和血质细胞是JDM中表达奥托费林的关键细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 儿科风湿病学 儿科风湿病学
背景情况:
- 青少年皮肤肌炎 (JDM) 是一种影响儿童的自身免疫性疾病.
- 奥托弗林在JDM病理生理学中的作用尚不清楚.
- 识别JDM疾病活动的生物标志物对于有效管理至关重要.
研究的目的:
- 评估奥托费林在JDM疾病中的作用.
- 评估未经治疗的JDM患者中奥托费林表达和疾病活性之间的关联.
- 在JDM中识别表达奥托费林的细胞.
主要方法:
- 定量实时PCR (qRT-PCR) 用于测量外围血液单核细胞 (PBMC) 和肌肉组织中的奥托费林mRNA表达.
- 流式细胞计测用于识别表达奥托费林的细胞.
- 在肌肉奥托费林表达的现场评估.
- 与疾病活动标志物的相关性分析.
主要成果:
- 与健康对照组相比,JDM患者的奥托费林mRNA表达显著更高 (p=0.001).
- 奥托费林表达与疾病活性得分 (DAS-总数,质蛋白,vWF:Ag) 有正相关,与CMAS.有负相关.
- 奥托费林主要表达在未切换的B细胞和血质细胞中.
结论:
- 在JDM中,奥托费林表达增加,并与疾病活动和肌肉衰弱有关.
- 奥托费林可能作为JDM疾病活动的潜在生物标志物.
- 在JDM中,B细胞和等离子体质细胞是奥托费林的主要细胞来源.
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