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在系统性硬化症中,IL-35的调节受损

Rubén Osuna-Gómez¹, Ivan Castellví², Maria Mulet¹

¹Inflammatory Diseases, Biomedical Research Institute Sant Pau (IIB Sant Pau), 08041 Barcelona, Spain.

International journal of molecular sciences

|July 14, 2023

概括

系统性硬化症 (SSc) 患者的IN-35 (IL-35) 水平较低,这与疾病的严重程度相关. 重组IL-35显示抗炎作用,表明其作为SSc.治疗点的潜力.

科学领域:

免疫学免疫学免疫学
类风湿病学类风湿病学
分子生物学分子生物学

背景情况:

系统性硬化症 (SSc) 是一种复杂的自身免疫性疾病,其特征是纤维化和血管异常.
特定的细胞因子,如INTERLEUKIN-35 (IL-35) 在SSc病原发生中的作用仍然不完全理解.
了解SSc中的免疫失调对于开发有效的治疗策略至关重要.

研究的目的:

研究系统性硬化症患者IL-35的水平和功能.
探索IL-35,T细胞反应和SSc.中的疾病严重程度标志物之间的关系.
评估IL-35作为SSc治疗点的潜力.

主要方法:

与酶相关的免疫吸收试验 (ELISA) 用于测量SSc患者和健康捐赠者的血IL-35,TGF-β和IL-17水平.
使用流式细胞计量来评估IL-35受体 (IL-35R) 在各种免疫细胞群中的表达.
再组合IL-35 (rIL-35) 被添加到刺激的外周血液单核细胞 (PBMC) 和Treg:Tresponder共同培养试验中,以评估其免疫调节作用.

主要成果:

与HD患者相比,SSc患者的IL-35度在血中显著降低 (52.1 ± 5.6对143 ± 11.1,p < 0.001).
IL-35水平与TGF-β (p < 0.001) 和IL-17 (p = 0.04) 有负相关性,与疾病严重程度 (mRSS,p = 0.004) 有正相关性.
在实验室中,rIL-35抑制了IL-17A和TGF-β的产生,并减少了T细胞的增殖,显示出抗炎性质.

结论:

IL-35在全身性硬化症中起着重要的免疫调节作用.
在SSc患者中降低IL-35水平与增加的炎症性细胞因子和疾病严重程度有关.
IL-35具有抗炎性质,是SSc.的潜在治疗标.

关键词:

CD4+ T 淋巴细胞介质蛋白-35 介质蛋白-35 监管T细胞的监管性T细胞系统性硬化症系统性硬化症

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