在Betula platyphylla中C-重复结合因子转录因子的全基因组识别和表达特征在非生物应激下
Xiang Zhang1, Jiajie Yu1, Ruiqi Wang1
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
International journal of molecular sciences
|July 14, 2023
概括
研究人员在Betula platyphylla中确定了20个C-重复结合因子 (CBF) 基因,揭示了它们在应对ABA,盐和寒冷等环境压力的作用. 这项研究增强了对木质植物中CBF转录因子的理解.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 无生物应激反应应激反应
背景情况:
- C-重复结合因子 (CBF) 转录因子对于植物非生物应激耐受性至关重要.
- 虽然在模型植物中进行了研究,但CBF基因在Betula platyphylla等木质物种中基本上没有表征.
研究的目的:
- 为了识别和表征贝图拉白的CBF基因亚家族.
- 研究BpCBFs在对非生物应激反应中的潜在作用.
主要方法:
- 对20个已识别的BpCBF基因进行生物信息分析,包括保存的域,基因结构和蛋白质基因.
- 与Arabidopsis thaliana和Populus trichocarpa进行比较的对线性分析.
- 在促进区域中识别 cis 作用元素.
- 在ABA,盐和冷应力下使用RNA-seq和qRT-PCR进行表达分析.
主要成果:
- 20个BpCBF基因被发现并进行了表征.
- 促进体分析揭示了与压力和荷尔蒙相关的 cis-acting 元素.
- 大多数BpCBF在应对ABA和盐应激时表达差异.
- 六种对冷反应的BpCBF被验证,显示在冷处理下升调.
- BpCBF7表现出转录激活活动.
结论:
- 这项研究提供了Betula platyphylla中的BpCBF基因家族的全面表征.
- BpCBF参与了对各种非生物压力的反应,包括ABA,盐和寒冷.
- 这项研究为木质植物中CBF转录因子的功能提供了宝贵的见解,并作为未来研究的基础.
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