转录组分析显示,在米饼中的5'UTR内由31bp的InDel触发的基因表达的变化
Qiuping Li1, Chunlong Zhang1, Jiancheng Wen1
1Rice Research Institute, Yunnan Agricultural University, Kunming 650201, China.
International journal of molecular sciences
|July 14, 2023
概括
一种31bp的InDel变异通过减少糖运输体3 (OsSUT3) 基因表达,显著影响米花粉的发育. 这影响了粉的积累和糖的代谢,这对植物繁殖至关重要.
科学领域:
- 植物生殖生物学 植物生殖生物学
- 分子遗传学 分子遗传学
- 植物生理学 植物生理学
背景情况:
- 花粉发育对于植物繁殖至关重要.
- 糖糖转运体3 (OsSUT3) 涉及到大米花粉的发展.
- 一个31bp插入/删除 (InDel) 变异影响下游基因表达.
研究的目的:
- 为了研究31bp InDel在花粉发育期间对基因表达的影响.
- 为了确定参与糖和粉代谢的基因受影响的InDel.
主要方法:
- 使用31bp的InDel结构 (p385-In/Del::OsSUT3-GUS) 构建和稳定转化转基因大米.
- 基因表达的基因化学和定量实时PCR (qRT-PCR) 分析.
- 在启动阶段对转基因大米饼的转录组概况.
主要成果:
- 删除31bp显著降低了OsSUT3和GUS基因表达在米饼.
- 转录组分析显示,InDel变体之间有1028个差异表达基因 (DEG).
- 与粉和糖代谢相关的19种DEG被确定为可能影响花粉中的粉积累.
结论:
- 31bp的InDel是下游基因表达的关键调节者,包括OsSUT3.3.
- 这种InDel影响了参与糖粉代谢的多个生物途径.
- 它为米粉中的糖代谢提供了一个新的调节机制,影响生育能力.
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