植物遗传转换的技术发展和应用
Wenbin Su1, Mingyue Xu1, Yasmina Radani1
1State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
International journal of molecular sciences
|July 14, 2023
概括
植物遗传转换增强了作物对环境挑战的特征. 本综述比较了Agrobacterium-mediated delivery等方法,并探索了再生技术,以提高转基因育种效率.
科学领域:
- 植物生物技术 植物生物技术
- 农业科学 农业科学
背景情况:
- 基因转换对于改善植物特征,如生物质和耐药性至关重要.
- 基因转换的进步促进了基因功能分析,特征利用和精确的育种.
研究的目的:
- 审查和比较当前的植物遗传转换技术.
- 讨论基因传递和植物再生的局限性和最近的进展.
主要方法:
- 直接 (由Agrobacterium介导) 和间接 (颗粒轰炸,电穿孔等) 的比较. 基因传递方法.
- 讨论再生途径:新生器官生成和体质胚胎生成.
- 探索形态遗传转录因子 (Bbm,Wus2,GRF-GIF) 进行增强的再生.
主要成果:
- 不同的基因转移方法具有不同的效率和局限性,包括基因型依赖和组织损伤.
- 特定转录因子的子宫外表达可以显著改善难以转化植物的再生.
- 优化策略正在出现,以克服物种和基因型特定的障碍.
结论:
- 了解不同植物遗传转换技术的原理,范围和局限性至关重要.
- 最近的进展为更高效和多功能转基因育种提供了优化的方法.
- 本综述为推进植物转基因育种策略提供了一个参考.
相关概念视频
Transgenic Plants
7.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.3K
Plant Breeding and Biotechnology
19.0K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.0K
Transgenic Organisms
31.3K
Overview
31.3K
Plant Tissue Culture
38.1K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
38.1K
Transformation
33
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
33
Recombinant DNA
95.9K
Overview
95.9K


