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来自Litopenaeus vannamei的曼诺斯受体通过病原体识别和炎症调节参与天生的免疫力

Na Guo^1,2, Yuan Liu^2,3, Qiang Hao^1,2

¹School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.

International journal of molecular sciences

|July 14, 2023

概括

太平洋白的曼诺酶受体 (LvMR) 识别病原体并与糖结合. 在细菌感染期间,LvMR作为炎症和反应性氧物种的负调节剂.

科学领域:

免疫学免疫学免疫学
海洋生物学海洋生物学
分子生物学分子生物学

背景情况:

曼诺酶受体 (MRs) 是C型莱克超级家族成员,参与模式识别和细胞信号传递.
太平洋白 (Litopenaeus vannamei) 是一个在商业上重要的水产养殖物种,面临着重大疾病挑战.

研究的目的:

在Litopenaeus vannamei.中识别和表征一个曼诺糖受体基因 (LvMR).
研究LvMR在的免疫识别和炎症反应中的功能.

主要方法:

基因鉴定和LvMR的序列分析.
复合蛋白表达和与PAMPs和碳水化合物结合的测试.
用RNA干扰进行基因淘汰实验.
分析免疫基因表达,ROS含量和感染后的iNOS活动.

主要成果:

LvMR具有特征域 (FN II,CRD) 和图案 (EPS,FND) 的特征.
LvMR转录物主要存在于肝胰腺中,并对病原体的挑战做出反应.
再组合LvMR以Ca2+依赖的方式结合PAMP和聚合微生物,对D-曼诺酸有很高的亲和力.
LvMR的淘汰会增加NF-κB通路基因,炎症和氧化还原基因,但不会增加细胞化基因.
在Vibrio parahaemolyticus感染后,LvMR敲除会提高ROS和iNOS活性.

结论:

在的免疫识别中,LvMR作为模式识别受体 (PRR) 起作用.
在的细菌感染期间,LvMR作为炎症反应的负调节剂.

关键词:

利托佩内亚斯 (Litopenaeus vannamei) 是一个水族群.这就是ROSOS ROS.这是一种炎症炎症炎症炎症.曼诺糖受体的受体为曼诺糖受体.病原体识别病原体识别

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