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药用植物Portulaca oleracea的遗传认证使用快速,精确和灵敏的同热DNA放大试验

Mo-Rong Xu¹, Fang-Chun Sun², Bo-Cheng Yang¹

¹Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.

International journal of molecular sciences

|July 14, 2023

概括

一种新的循环介导同热放大 (LAMP) 试验准确地识别了Portulaca oleracea (PO) 的DNA. 这种快速的方法可以检测草药产品中的伪造物,确保这种重要的药用作物的质量.

科学领域:

植物学植物学
分子生物学分子生物学
药理学是指药理学,即药理学是指药理学.

背景情况:

葡萄 (Portulaca oleracea,PO) 是传统中医药 (TCM) 中的一种有价值的药用作物.
PO经常与相关物种混杂,从而损害了其身份和有效性.
准确的植物认证对于制药和功能性食品的应用至关重要.

研究的目的:

开发一种特定的,灵敏的,快速的基于DNA的测定方法来验证Portulaca oleracea (PO) 的真实性.
评估来自草药市场的商业PO样本中改的普遍性.

主要方法:

设计和验证针对ITS2序列的循环介导同热放大 (LAMP) 启动器.
优化LAMP测试条件 (温度,时间).
测试测试的特异性,灵敏度和适用于热处理和商业PO样本的适用性.

主要成果:

开发的LAMP试验在同热条件下 (温度为63°C30分钟) 实现了PO DNA的100 fg的检测极限.
该测试表明PO DNA认证的特异性和敏感性.
对商业PO样本的分析显示,在草药市场中约有68.4%的改.

结论:

一种新的,快速的,特定的和敏感的LAMP测定方法用于 Portulaca oleracea (PO) 认证已经成功开发出来.
该测试对于收获后的认证和在商业PO产品中检测伪造具有实用性.
这种工具将有助于验证PO及其衍生产品的植物起源.

关键词:

葡萄兰 (Portulaca oleracea) 是一个植物.认证的真实性认证的真实性循环介导的同热放大 (LAMP)药用作物药用作物药用作物传统中国医学 (TCM) 是一种

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