光子和单片氧诱导的水溶性胡卜素克罗辛的Cis-Trans异构
Franco Fusi1, Giovanni Romano1, Giovanna Speranza2
1Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Viale G. Pieraccini, 6, 50139 Florence, Italy.
研究了素 (CR) 异构,这对于理解cis-carotenoid作用和抗氧化潜力至关重要. cis-to-trans反应显著更有效,这表明CR可以在体内灭活性氧物种.
科学领域:
- 生物化学 生物化学
- 摄影化学的使用.
- 胡卜素化学 胡卜素化学
背景情况:
- 沙夫兰中的水溶性胡卜素crocin (CR) 在体内发挥作用.
- 了解 cis-trans 异构化是其生理功能和抗氧化剂应用的关键.
研究的目的:
- 研究crocin的 cis-trans 异构化动力学.
- 确定由光或单片氧诱导的异构化效率.
- 通过研究其反应性氧物种灭能力,评估crocin作为抗氧化剂的潜力.
主要方法:
- 在甲醇和水中诱导crocin的cis-trans异构化,使用光或单片氧.
- 使用高性能液态染色学 (HPLC) 监测分子度.
- 采用非破坏性光谱法测量方法来追踪异构化,漂白和单片氧气水平.
主要成果:
- 开发了一种全面的模型,用于从三元体状态进行素异构,而不论激发方法 (光子或单元氧).
- 与trans-to-cis相比,cis-to-trans同质化效率显著更高 (1.9到10倍) 的表现.
- 观察到一个不可忽视的单点氧诱导漂白作用在水中的crocin.
结论:
- 从cis到trans的crocin异构化更有效,支持其在生物系统中的作用.
- 这种"翻转"的异构化机制表明,crocin可以在体内有效地灭活性氧物种 (ROS).
- 这一过程可能提供一种治疗机制,对胡卜素再生的需求最小.
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