NIH/3T3 纤维细胞选择性地激活特异于翻译后修改的 II 型原蛋白的 T 细胞
Balik Dzhambazov1, Tsvetelina Batsalova1, Patrick Merky2
1Faculty of Biology, Paisii Hilendarski University of Plovdiv, 4000 Plovdiv, Bulgaria.
International journal of molecular sciences
|July 14, 2023
概括
NIH / 3T3纤维细胞激活T细胞混合体,识别类风湿性关节炎自身抗原,即使没有抗原存在. 这表明了类风湿性关节炎 (RA) 发病过程中涉及纤维细胞-TCR相互作用的新机制.
科学领域:
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
- 细胞生物学 细胞生物学
背景情况:
- 突纤维细胞 (SF) 参与了类风湿性关节炎 (RA) 的发病,可能是通过呈现抗原.
- 纤维细胞可以表达MHC II类 (MHCII) 分子,使免疫细胞能够呈现抗原.
研究的目的:
- 调查各种纤维细胞类型是否可以处理和呈现II型原体 (COL2),一种RA自身抗原.
- 确定NIH/3T3纤维细胞在启动RA病原和T细胞激活中的作用.
主要方法:
- 使用的MHCII/Aq受限制的T细胞杂交瘤系,特定于一个COL2表位 (COL2259-273).
- 通过突细胞,皮肤细胞,胸膜细胞,LS48和NIH/3T3纤维细胞评估T细胞克隆的激活.
- 研究了涉及T细胞受体 (TCR) 综合体的抗原独立激活机制.
主要成果:
- NIH/3T3 纤维细胞激活了 T 细胞克隆,识别了经过翻译修改的 COL2259-273.
- 与其他纤维细胞不同的是,NIH / 3T3细胞刺激了特定于糖化COL2259-273 (Gal264) 的混合体.
- 通过依赖接触的TCR机制,NIH/3T3纤维细胞激活了没有外源性COL2或改性的COL2特异性T细胞杂交瘤.
结论:
- NIH/3T3纤维细胞具有独特的能力,可以激活特定的T细胞杂交瘤,而不依赖于抗原的存在.
- 这种抗原独立的激活机制可能会导致类风湿性关节炎的开始.
- 纤维细胞-TCR相互作用代表了自身免疫性疾病发病的潜在途径.
相关概念视频
Introduction to Fibroblasts
3.1K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.1K
Fibril-associated Collagen
2.6K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.6K
Phases of Wound Repair
6.1K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
6.1K
Type IV Collagen of Basal Lamina
2.3K
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...
A type IV collagen molecule has six alpha chains which can...
2.3K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
Collagens are the Major Structural Proteins of ECM
4.3K
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. Collagen fiber is made from fibrous protein subunits linked together to form a long, straight fiber. Collagen fibers, while flexible, have great tensile strength, resist stretching, and give ligaments and tendons their characteristic resilience and strength. These fibers hold connective tissues together, even during the body's movement.
Connective tissue proper includes loose...
Connective tissue proper includes loose...
4.3K


