对抗植物病原性细菌的新型7-阿利法氨基三氨基衍生物的发现和机制
Xuesha Long1, Guanglong Zhang2, Haitao Long1
1School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China.
International journal of molecular sciences
|July 14, 2023
概括
一种新化合物6e显示出作为农业化学杀菌剂的强大潜力. 它有效地对抗由Xanthomonas oryzae pv引起的水细菌叶病. (Xoo),保护作物和提高产量.
科学领域:
- 农业化学 农业化学
- 植物病理学 植物病理学
- 有机合成 有机合成
背景情况:
- 大米细菌叶病,由Xanthomonas oryzae pv.v.引起的 (Xoo),对全球大米生产和粮食安全构成重大威胁.
- 现有的杀菌剂面临着有效性和环境影响的挑战,需要开发新的抗菌剂.
研究的目的:
- 设计,合成和评估新型的7-阿利法氨基三氨酸衍生物作为潜在的杀菌剂对抗Xoo.
- 为了研究最强大的衍生品的抗菌作用机制.
主要方法:
- 合成了一系列的7-阿利法性氨基三氨酸衍生物.
- 检测Xoo的体外抗菌活性,确定EC50值.
- 在受感染的米植物上进行体内疗效测试.
- 扫描电子显微镜 (SEM) 观察细菌形态变化.
- 细胞内活性氧物种 (ROS) 积累和生物膜形成的分析.
主要成果:
- 与商用杀菌剂相比,化合物6e在Xoo (EC50 = 2.55μg/mL) 的体外活性更强.
- 在体内研究证实了6e对大米叶的显著保护作用.
- 在使用6e治疗后,SEM揭示了Xoo细菌细胞的部分缩.
- 6e诱导了细胞内ROS的积累,导致细菌细胞亡并抑制生物膜的形成.
结论:
- 合成的7-阿利法氨基三氨基衍生物6e是对抗大米细菌叶病的高度有效剂.
- 化合物6e表现出一个有前途的作用机制,涉及ROS生成和亡诱导.
- 6e代表了一种潜在的新型农化杀菌剂,用于在米种植中可持续管理Xoo.
相关概念视频
01:26Physical Properties of Amines
3.2K
Amines with low molecular weight are usually gaseous at room temperature, while those with high molecular weight are liquid or solids in nature. Usually, low molecular weight amines have a rotten fish-like smell. Diamines typically have a pungent smell. For instance, cadaverine and putrescine, depicted in Figure 1, are two molecules responsible for decaying tissue.
3.2K
01:07Amines: Introduction
4.5K
Amines are organic derivatives of ammonia. They are formed by replacing one or more ammonia protons with alkyl or aryl groups. Depending upon the number of organyl groups bonded to nitrogen, amines are classified as primary, secondary, or tertiary. Primary amines have one organyl group attached to the nitrogen atom, while secondary and tertiary amines have two and three organyl groups attached to the nitrogen atom, respectively.
4.5K
01:11Aryldiazonium Salts to Azo Dyes: Diazo Coupling
3.0K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...
3.0K
01:07Preparation of 1° Amines: Hofmann and Curtius Rearrangement Overview
3.2K
In the presence of an aqueous base and a halogen, primary amides can lose the carbonyl (as carbon dioxide) and undergo rearrangement to form primary amines. This reaction, called the Hofmann rearrangement, can produce primary amines (aryl and alkyl) in high yields without contamination by secondary and tertiary amines.
3.2K
01:19Structure of Amines
2.6K
The hybridized nitrogen atom in amines possesses a lone pair of electrons and is bound to three substituents with a bond angle of around 108°, which is less than the tetrahedral angle of 109.5°. However, the C–N–H bond angle is slightly larger at 112°, with a carbon–nitrogen bond length of 147 pm. This carbon–nitrogen bond length of of amines is longer than the carbon–oxygen bond of alcohols (143 pm) but shorter than alkanes’...
2.6K
01:23Antimicrobial Proteins
1.1K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
1.1K