在COVID-19患者的唾液miRNA概况不同疾病严重程度的患者
Irma Saulle1,2, Micaela Garziano1,2, Gioia Cappelletti2
1Department of Pathophysiology and Transplantation, University of Milan, Via Francesco Sforza, 20122 Milan, Italy.
International journal of molecular sciences
|July 14, 2023
概括
这项研究研究了唾液和血中的SARS-CoV-2感染标志物. 严重的COVID-19患者表现出明显的microRNA (miRNA) 变化和炎症性细胞因子的增加,与疾病严重程度和免疫激活相关.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 口腔粘膜是SARS-CoV-2进入和复制的主要部位.
- 了解唾液和血中的早期免疫反应对于管理COVID-19至关重要.
研究的目的:
- 分析SARS-CoV-2病毒载量,微RNA (miRNA),细胞因子和中和活性 (NA) 在轻度 (MD) 和重度 (SD) COVID-19患者中.
- 为了确定潜在的生物标志物来预测疾病的严重程度和进展.
主要方法:
- 从MD和SDCOVID-19患者的唾液和血中分析了84个miRNAs.
- 预炎性细胞因子和化学因子的量化.
- 评估中和活性 (NA) 和免疫激活标志物.
主要成果:
- 在SD患者的血和唾液中,有8种miRNA被差异表达,其中特定的let-7家族miRNA被调低,其他则被调高.
- 在MD和SD患者中观察到,促炎性细胞因子 (IL-1β,IL-2,IL-6,IL-8,IL-9,TNFα) 和化学因子 (CCL2,RANTES) 的产生增加.
- 疾病严重程度与NA和免疫激活标志物有显著的相关性.
结论:
- 特定的miRNA表达模式和唾液和血中细胞因子/化学因子水平的升高与COVID-19的严重程度有关.
- 这些参数,包括NA,可以作为预测疾病结果和进展的有价值的生物标志物.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Coronavirus
Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...
Respiratory Syncytial Virus Disease
Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...


