在多种组织和发育阶段对猪的染色质可访问性进行分析
Jingyi Bai1, Yu Lin1, Jiaman Zhang1
1Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
International journal of molecular sciences
|July 14, 2023
概括
本研究使用ATAC-seq.绘制新生猪组织和发育中的肝脏中染色质可访问性的地图. 研究结果揭示了开放色素如何调节组织特异性基因和肝脏发育,有助于生物医学和农业应用.
科学领域:
- 表观遗传学和基因组学
- 哺乳动物发育生物学
- 转录条例 转录条例 转录条例
背景情况:
- 了解染色质可访问性对于破译不同组织和发育阶段的基因调节至关重要.
- 关于新生猪和猪肝发育期间的染色质可访问性的数据有限.
研究的目的:
- 为新生猪的多种组织生成全面的染色质可访问性地图.
- 调查猪肝发育过程中染色质可访问性的动态变化.
- 确定与组织特异性功能和肝脏成熟相关的调控元素.
主要方法:
- ATAC-seq (使用测序测定转移酶可访问染色体的测试) 用于对开放染色体进行分析.
- 用rRNA枯竭的RNA-seq来分析转录特征.
- 分析包括蛋白质编码基因 (PCG),长非编码RNAs (lncRNAs) 和转录复杂的独特编码潜在转录 (TUCPs).
主要成果:
- 特定组织可访问的促进剂与与专门组织功能相关的蛋白质编码基因的活跃转录有关.
- 非编码组织特异性峰值与身体活动和新生儿组织形态发生有关.
- 动态色素可访问性变化与出生后肝脏发育期间的肝功能过渡相关.
结论:
- 这项研究提供了有关猪组织和肝脏发育的染色质可访问性的详细资料.
- 这些发现增强了对管理哺乳动物组织和器官发育的表观遗传机制的理解.
- 这些数据可以为改善猪经济特征和生物医学应用的战略提供信息.
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