相关实验视频

Updated: Jul 23, 2025

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

EbbHLH80通过上调调类积累和调节ROS水平来增强盐的反应

Qingqing Gao^1,2, Xia Li^1,2, Chunfan Xiang^1,2

¹National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China.

International journal of molecular sciences

|July 14, 2023

概括

埃里格伦皮树bHLH80 (EbbHLH80) 基因增强了植物对盐的耐受性. EbbHLH80通过调节反应性氧物种清理和增加应激反应基因来促进盐分耐受性.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

基本的螺旋环-螺旋环 (bHLH) 转录因子调节植物非生物应激反应.
艾瑞格龙 (Erigeron breviscapus) 产生的黄类药物对心血管有好处.
来自E. breviscapus的基因EbbHLH80积极调节黄类化合物合成.

研究的目的:

为了研究EbbHLH80在盐应激反应中的功能.
为了确定EbbHLH80是否增强了黄类积累和盐分耐受性.
阐明了EbbHLH80介导的盐耐受性背后的分子机制.

主要方法:

对EbbHLH80.80的遗传学分析
对EbbHLH80过度表达 (OE) 和野生型 (WT) 烟草种子的代谢和转录组分析.
评估转基因烟草在发芽和苗木生长期间的盐耐受性.
测量活性氧物种 (ROS) 水平和抗氧化酶表达.

主要成果:

在盐处理下,EbbHLH80的表达增加.
与WT相比,OE植物表现出较强的盐耐受性.
转基因烟草在盐应激后表现出较低的ROS水平和增加的抗氧化酶活性.
鉴定了与黄类生物合成和盐应激相关的基因和代谢物.

结论:

EbbHLH80 作为植物盐分耐受性的积极调节剂.
EbbHLH80通过调节ROS清理和应激反应基因来促进盐分耐受性.
EbbHLH80可能会增强黄类积累,有助于改善应激弹性.

关键词:

这就是为什么 EbbHLH80H80 埃里杰伦的短视 (Erigeron breviscapus) 是一个这就是ROSOS ROS.黄类化合物黄类化合物盐的耐受性盐的耐受性压力反应基因的基因.

更多相关视频

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

相关概念视频

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.