阿片类药物通过Nrf2/HO-1途径在LPS刺激的微质中减轻氧化压力
Akash Shivling Mali1, Ondrej Honc1, Lucie Hejnova1
1Department of Physiology, Faculty of Science, Charles University, 12800 Prague, Czech Republic.
International journal of molecular sciences
|July 14, 2023
概括
阿片类受体激动剂在微质细胞中表现出抗氧化和抗炎作用. 这些化合物通过保护线粒体功能和减少活性氧物种 (ROS) 来保护氧化应激.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 阿片类药物具有抗氧化特性,可以调节微质功能.
- 阿片类药物配体抑制促炎性细胞因子的释放,并促进抗炎性微质极化.
- 脂聚糖 (LPS) 是一种常见的刺激剂,用于激活微质炎症反应.
研究的目的:
- 研究特定阿片类受体激动剂 (DAMGO,DADLE,U-50488) 的抗炎和抗氧化潜力.
- 检查这些激动剂对LPS激活的小鼠微质细胞 (C8-B4细胞系) 的作用.
主要方法:
- 使用的C8-B4小鼠微质细胞被脂聚糖 (LPS) 激活.
- 评估了阿片类受体激活剂 (DAMGO,DADLE,U-50488) 对细胞反应的影响.
- 测量了反应性氧物种 (ROS) 生产,NADPH合成,葡萄糖吸收,降低的谷氨水平,ATP含量和线粒体呼吸.
- 研究了Nrf2/HO-1信号通路的参与.
主要成果:
- 所有测试的阿片类药物激动剂都产生了针对LPS诱导的氧化应激的细胞保护作用.
- 阿片类药物减少了LPS诱导的ROS产生,调节了NADPH合成,并影响了葡萄糖的摄取.
- 阿片类药物增加了减少的谷氨水平,ATP含量,并增强了线粒体呼吸.
- 与Nrf2/HO-1通路的上调相关的有益作用.
结论:
- 阿片类药物信号激活保留了线粒体功能,并消除了微质中的ROS.
- Nrf2/HO-1信号通路调解了微质中阿片类药物的抗氧化疗效.
- 阿片类受体激动剂显示出抑制微质氧化应激和炎症的治疗剂的潜力.
关键词:
它们是NADPH,NADPH.Nrf2 / HO-1 的一个.葡萄糖运输体是葡萄糖的运输体.脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.微质细胞中的微质细胞在阿片类药物阿片类药物.氧化应激是一种氧化应激.更多相关视频
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