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血清循环的microRNAs在零星的包括身体肌肉炎

Matteo Lucchini^1,2, Valeria De Arcangelis¹, Massimo Santoro³

¹UOC Neurologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.

International journal of molecular sciences

|July 14, 2023

概括

研究人员在患有偶发性包容体肌炎 (s-IBM) 的患者血液中确定了特定的microRNAs (miRNAs). 这些循环的miRNAs可能作为这种罕见的自身免疫和退行性肌肉疾病的生物标志物.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
神经学神经学

背景情况:

零星包容体肌肉炎 (s-IBM) 是一种复杂的异常性炎症性肌肉炎症,具有退行性和自身免疫性方面.
目前对s-IBM的治疗方法有限,强调需要更好地了解其病理生理学.
循环微RNAs (miRNAs) 是各种疾病的潜在生物标志物,为疾病机制提供了洞察力.

研究的目的:

研究s-IBM患者血清中特定循环miRNA的表达特征.
为了识别潜在的血清miRNA生物标志物用于s-IBM诊断和了解疾病机制.

主要方法:

从14名s-IBM患者和8名年龄和性别匹配的健康对照人群中采集了血清样本.
使用定量实时PCR分析了预放大miRNAs的表达水平.
为了分析miRNA表达数据,采用了两种不同的规范化方法.

主要成果:

一个miRNA (hsa-miR-192-5p) 与对照组相比,在s-IBM患者中显著下调.
三种miRNA被上调,hsa-miR-372-3p显示s-IBM患者的显着增加.
在s-IBM血清中,hsa-miR-192-5p和hsa-miR-372-3p被确定为显著失调.

结论:

在s-IBM患者中hsa-miR-192-5p和hsa-miR-372-3p的失调表达表明它们参与了疾病病理生理学.
这些与分化和再生过程相关的特定miRNA可能反映了s-IBM肌肉中的病理机制.
hsa-miR-192-5p和hsa-miR-372-3p代表了零星包含体肌炎的有希望的候选生物标志物.

关键词:

这就是hsa-miR-192-5pp.这就是hsa-miR-372-3p.异形性炎症性肌肉病变 (Idiopathic Inflammatory Myopathy) 是一种异形性炎症性肌肉病变 (Idiopathic Inflammatory Myopathy) 是一种异形性炎症性肌肉病变.包括体肌肉炎的包括体肌肉炎这是一个小RNARNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...