在HPLC-MS/MS分析人类血样本中的异构体识别,使用由基于线性方程的算法支持的离子陷
Marco Pallecchi1, Luigi Lucio1, Laura Braconi1
1Neurofarba Department, Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, Polo Scientifico, Via U. Schiff 6, Sesto Fiorentino, 50019 Firenze, Italy.
International journal of molecular sciences
|July 14, 2023
概括
这项研究提出了一种新的液体染色学-并联质谱法 (LC-MS/MS) 方法用于同位素识别. 优化的方法使用先进的数据处理有效地区分人体血中的异构体,提高分析能力.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 在各种科学领域,同位素识别至关重要.
- 传统的异构体分析方法可能是复杂和耗时的.
- 双重质谱法 (MS/MS) 提供了异构体差异化的潜力.
研究的目的:
- 开发和验证用于同位素识别的快速液体染色学-并联质谱法 (HPLC-MS/MS) 方法.
- 为了优化MS/MS参数,以便有效地分配异构体信号.
- 应用开发的方法来分析人体血样本中的异构体.
主要方法:
- 使用离子陷并联质谱仪 (IT-MS/MS) 用作为碰撞气体.
- 采用液态染色法 (HPLC),不需要对同位素进行基线分离.
- 使用后处理数据处理 (LEDA) 和调整激发幅度 (ExA) 和激发时间 (ExT) 的优化MS/MS属性.
- 实施了两次分段采购策略,以缩短循环时间和改进染色图谱.
主要成果:
- 开发的HPLC-MS/MS方法成功地在没有先前染色学分辨率的情况下区分异构体.
- 优化IT-MS/MS参数,包括ExA和ExT,促进了异构体特定的碎片化.
- 该方法在分析高度人体血样本方面表现出效率,证实了其适用性.
- 两段时间的方法有效地减少了采集时间,并保持了色谱完整性.
结论:
- 拟议的HPLC-MS/MS方法为同位素识别提供了一个简单,快速和有效的解决方案.
- 该方法克服了传统的MS/MS技术的局限性,因为它可以在没有完全染色学分离的情况下实现异构体的分化.
- 这种技术对复杂的生物基质如人体等离子体中同位素的常规分析具有前景.
更多相关视频
相关概念视频
High-Resolution Mass Spectrometry (HRMS)
1.4K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.4K
Mass Spectrometry: Complex Analysis
828
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
828
Peptide Identification Using Tandem Mass Spectrometry
6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Electrospray Ionization (ESI) Mass Spectrometry
940
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
940
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
524
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
524
High-Performance Liquid Chromatography: Introduction
2.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.1K


