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转录因子 Nrf2 调节牛子宫内膜上皮细胞中脂聚糖诱导的损伤

Pengjie Song¹, Chen Liu¹, Mingkun Sun¹

¹Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China.

International journal of molecular sciences

|July 14, 2023

概括

在牛子宫内膜炎期间,核因子-氨酸-2-相关因子2 (Nrf2) 和内质网膜应激 (ERS) 被激活. Nrf2通过激活ERS中的PERK通路来促进炎症和亡.

科学领域:

兽医免疫学兽医免疫学
分子生物学分子生物学
细胞病理学细胞病理学

背景情况:

乳牛的子宫内膜炎会对乳生产和乳业经济产生负面影响.
牛子宫内膜上皮细胞 (BEECs) 中的细胞内膜网应激 (ERS) 与产后压力因素的炎症有关.
核因子-红色素-2-相关因子2 (Nrf2) 是炎症反应的关键调节者,但其在ERS介导炎症中的作用尚不清楚.

研究的目的:

调查Nrf2在BEECs中脂聚糖 (LPS) 诱导的损伤中的作用.
阐明Nrf2在ERS和炎症中的参与背后的分子机制.

主要方法:

在牛子宫内膜炎患者中分析Nrf2和ERS基因表达.
用LPS刺激孤立的BEEC来模仿炎症状况.
使用Nrf2敲击来评估其对炎症和ERS标志物的影响.

主要成果:

在子宫内膜炎病例和用LPS治疗的BEEC中,nrf2和ERS基因表达升高.
Nrf2激活增强了炎症标记物 (TNF-α,p65,IL-6,IL-8) 和ERS标记物 (GRP78,PERK,eIF2α,ATF4,CHOP) 的表达.
Nrf2敲击降低了炎症和ERS标志物表达,并降低了亡.

结论:

在BEEC炎症期间,Nrf2和ERS同时被激活.
Nrf2通过激活ERS.内的PERK通路来加剧BEEC的炎症和亡.

关键词:

Nrf2 没有任何问题.牛的子宫内膜炎牛的子宫内膜炎细胞内膜网膜的压力应激这是一种炎症炎症炎症炎症.

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