通过液体染色学 (Tandem Mass Spectrometry) 在干中测定农药的多残留方法
Marcin Gruba1, Emilia Jóźwik1, Mariusz Chmiel1
1Łukasiewicz Research Network-New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13a, 24-110 Puławy, Poland.
Molecules (Basel, Switzerland)
|July 14, 2023
概括
一种新的方法使用QuEChERS和Z-Sep+ sorbent准确地确定了干麻中的36种农药残留物. 这种经过验证的方法可以确保对蜂蜜产品进行可靠的农药分析.
科学领域:
- 农业化学 农业化学
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
背景情况:
- 干燥的蜂是农业的关键商品.
- 准确的农药残留分析对于食品安全和质量控制至关重要.
- 现有的在蜂中测定农药的方法需要提高效率和准确性.
研究的目的:
- 开发和验证一种多种残留方法,用于在干中确定36种农药.
- 使用QuEChERS (快速,轻松,便宜,坚固和有效) 方法优化样本准备.
- 评估各种分散固相提取 (dSPE) 吸附剂的有效性,包括Z-Sep+,用于矩阵清洁.
主要方法:
- 使用乙酸缓冲的QuEChERS方法进行样本提取.
- 研究PSA,C18,GCB,Z-Sep和Z-Sep+吸附剂的混合物用于dSPE清洁.
- 分析样品使用液态色谱学与合质谱学 (LC-MS/MS) 进行分析.
- 根据SANTE/11813/2017指南验证了该方法.
主要成果:
- 含有Z-Sep+的吸附剂混合物显示出更高的清洁效率,最大限度地减少了共提取剂.
- 该方法实现了所有农药的量化限值 (LOQ) 在0.02-0.1 mg/kg之间.
- 方法验证显示真实性 (恢复) 从70-120%和精度 (RSDr) <20%.
- 经过验证的方法已成功应用于分析15个真正的干蜂蜜样本.
结论:
- 开发的采用Z-Sep+ dSPE的QuEChERS方法对于在干燥的蜂中测定多种残留农药是有效的.
- 这项研究代表了Z-Sep+吸附剂在蜂蜜分析中的首次应用,并改进了现有的杀虫剂残留物确定方法.
- 经过验证的方法提供了一种可靠的工具,用于定期监测干蜂中的农药残留物,确保产品的安全.
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