使用混合石墨烯基静止相的毛细液-LC柱交换系统的适用性和局限性
João Victor Basolli Borsatto1,2, Edvaldo Vasconcelos Soares Maciel1,3, Alejandro Cifuentes2
1Laboratory of Chromatography, Institute of Chemistry at Sao Carlos, University of Sao Paulo, P.O. Box 780, Sao Carlos 13566590, Brazil.
Molecules (Basel, Switzerland)
|July 14, 2023
概括
这项研究展示了一种基于石墨烯的列切换系统,用于分析软饮料中的pyriproxyfen. 虽然该系统对pyriproxyfen有效,但对abamectin B1a分析显示了局限性.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 氧化石墨烯在二氧化 (SiGO) 和C18-endcapped SiGO (SiGO-C18ec) 是已确立的液态染色学 (LC) 的吸收剂.
- 列切换系统在染色学中提供了更高的样本准备和分析效率.
研究的目的:
- 为了评估一个专交换系统,使用完全基于石墨烯的吸附剂 (SiGO提取,SiGO-C18ec分析).
- 评估该系统在味软饮料中的pyriproxyfen和abamectin B1a分析的适用性和局限性.
主要方法:
- 使用SiGO作为提取柱和SiGO-C18ec作为分析柱构建了一个柱交换系统.
- 在一个色风味碳酸软饮料矩阵中分析了pyriproxyfen和abamectin B1a.
主要成果:
- 该系统成功量化了pyriproxyfen (0.5-25μg/mL),具有出色的线性 (R2=0.9931) 和良好的精度 (82.2-111.4%).
- 观察到矩阵效应,较高的汁含量增强了pyriproxyfen信号;SiGO提取列显示了120次注射的寿命.
- 该系统不适合对阿巴麦克B1a的分析,SiGO-C18ec列与标准C18列相比显示出显著的峰值尾部.
结论:
- 基于石墨烯的列切换系统可用于复杂矩阵中的特定分析物分析,如pyriproxyfen.
- 对于某些分析物 (例如,阿巴麦B1a) 存在限制,并且列性能 (例如,对SiGO-C18ec的尾巴) 需要进一步调查.
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