基于指纹和化学测量的Codonopsis Radix的质量评估和分类
Xuxia Liu1, Zhengjun Chen1, Xin Wang1
1School of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730013, China.
Molecules (Basel, Switzerland)
|July 14, 2023
概括
这项研究使用HPLC指纹和化学测量来评估Codonopsis Radix (CR) 的质量. 来自甘的CR和传统制加工显示出最佳质量,突出了它们在药用中的重要性.
科学领域:
- 药理学是指药理学,即药理学是指药理学.
- 分析化学 分析化学
- 化学测量 化学测量 化学测量
背景情况:
- 在中国,Codonopsis Radix (CR) 被广泛用于食品和药品.
- 了解影响CR质量的因素,如原产地,储存和加工,至关重要.
研究的目的:
- 根据原产地,储存时间和制加工,制定评估CR质量的综合战略.
- 识别标记化合物并评估CR样本中的含量变化.
主要方法:
- 使用二极管阵列检测 (HPLC-DAD) 的高性能液态色谱,用于指纹.
- 化学测量方法:相似性分析 (SA),主要成分分析 (PCA),层次聚类分析 (HCA) 和O2PLS-DA.
- 质谱 (MS) 用于化学成分的识别.
主要成果:
- 来自甘省的CR显示出最高质量,其次是山西和四川. 重庆和贵州的样本质量不佳.
- 当新鲜加工 (0年储存) 和传统熟时,CR质量是最佳的,这表明了这一过程的重要性.
- 鉴定出了13种和16种标记化合物,分别根据来源,储存和加工来区分CR. 洛贝提林含量有显著的变化.
结论:
- HPLC指纹与化学分析相结合,提供了一种可靠的方法来区分CR原材料.
- 这种方法对于Codonopsis Radix的质量控制和评估是有效的.
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