来自阿斯特拉加勒斯菌的多糖体的结构特征和免疫生物学活性
Qiuxia Meng1, Yu Niu2, Rongrong Wang3
1Institute of Eco-environment and Industrial Technology, Shanxi Agricultural University, Taiyuan 030031, China.
Molecules (Basel, Switzerland)
|July 14, 2023
概括
补充菌基质与阿斯特拉加勒斯根改变了多糖结构和增强了免疫生物学的活性. 这些来自阿斯特拉加勒斯的多糖体显示出作为中性药物的潜力.
科学领域:
- 菌类学和生物化学 菌类学和生物化学
- 免疫学和药理学
背景情况:
- 可食用和药用草药,如阿斯特拉加勒斯根,可以修改培养的的营养和药用特性.
- 是一种有价值的食物来源,具有潜在的健康益处.
研究的目的:
- 为了提取和表征菌的多糖,从菌生长在阿斯特拉加勒斯补充基板上.
- 为了比较这些多糖的免疫生物活动,并阐明它们的作用机制.
主要方法:
- 超声波辅助热水提取,Sevage方法脱蛋白化,H2O2脱色,透析和柱状染色学 (DEAE-52纤维素,Sephadex G-100) 用于多糖类净化.
- 分析了RAW264.7细胞上的单糖组成,分子量和免疫调节作用 (NO,TNF-α,细胞毒性,ROS).
- 参与免疫调节的信号通路 (JNK/MAPK,Erk/MAPK,NF-κB) 在体外进行了研究.
主要成果:
- 两个多糖类P0OP-I (0% Astragalus) 和P15OP-I (15% Astragalus) 分离出来,分别具有21,706.96Da和20,172.65Da的分子量.
- 这两种多糖都包括D-曼诺斯,银酸,D-葡萄糖,D-银糖和L-阿拉比诺斯. 与P0OP-I相比,P15OP-I具有更高比例的曼诺,葡萄糖,银和阿拉比诺,以及较低的银酸.
- 两种多糖都在RAW264.7细胞中诱导了氧化 (NO) 和瘤亡因子-α (TNF-α) 的产生,而没有细胞毒性或反应性氧物种 (ROS) 的产生. P15OP-I表现出更强的免疫调节活性.
- 免疫调节作用通过JNK/MAPK,Erk/MAPK和NF-κB信号通路进行调节.
结论:
- 阿斯特拉加勒斯补充剂改变了的多糖化合物组成.
- 由此产生的多糖类具有免疫调节特性,其中P15OP-I具有增强的活性.
- 这些发现支持了阿斯特拉加勒斯修饰菌作为免疫健康中性药物的来源的潜力.
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