相关实验视频
Updated: Jul 23, 2025
10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
24.4K
在Lycoris chinensis中建立病毒诱导的基因沉默系统
Guanghao Cheng1,2,3, Xiaochun Shu1,2,3, Zhong Wang1,2,3
1Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
Plants (Basel, Switzerland)
|July 14, 2023
概括
研究人员开发了一种有效的病毒诱导基因沉默 (VIGS) 系统,用于使用叶尖注射的Lycoris植物. 该研究发现,Lycoris CLA1 (LcCLA1) 是比LcPDS更有效的基因沉默指标.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 百合花种类具有重要的药用和装饰价值.
- 缺乏有效的基因转换系统阻碍了Lycoris中的基因功能研究.
- 病毒诱导的基因沉默 (VIGS) 是植物基因功能分析的一个有价值的工具.
研究的目的:
- 建立一个有效的病毒诱导基因沉默 (VIGS) 系统,用于中国.
- 评估Lycoris CLA1 (LcCLA1) 和Lycoris PDS (LcPDS) 作为VIGS视觉指标的疗效.
- 为了优化具有状叶面的单植物中的基因沉默.
主要方法:
- 对VIGS的烟草虫病毒 (TRV) 载体的构建.
- 选择和应用LcCLA1和LcPDS基因作为记者基因.
- 叶尖针注射方法用于将TRV输入Lycoris叶子.
- 定量逆转录PCR (qRT-PCR) 用于评估基因表达水平.
主要成果:
- 通过TRV介导的VIGS成功诱导了Lycoris叶的可见黄色 (色).
- 与LcPDS相比,LcCLA1沉默导致更明显和更广泛的化.
- qRT-PCR证实了LcCLA1和LcPDS的显著下调,LcCLA1.1的沉声效率更高.
- 叶尖注射方法被证明是有效的,可以提高VIGS在状单角叶的效率.
结论:
- 一个功能性VIGS系统成功地建立了Lycoris chinensis.
- 鉴于其更高的沉默效率,LcCLA1被确定为Lycoris中的VIGS更适合的记者基因.
- 开发的VIGS系统和叶尖注射方法将促进未来在Lycoris物种的基因功能研究.
相关概念视频
02:30siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
02:15Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
01:23RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K