维生素K2是否会影响大鼠模型中糖尿病和椎间盘退化之间的相互作用?
Mohamed Mahmoud1, Maria Kokozidou1, Clemens Gögele1
1Institute of Anatomy and Cell Biology, Paracelsus Medical University, Nuremberg and Salzburg, Prof. Ernst Nathan Str. 1, 90419 Nuremberg, Germany.
Nutrients
|July 14, 2023
概括
维生素K2可能会影响2型糖尿病中椎间盘 (IVD) 退化. 虽然它影响了糖尿病老鼠IVD细胞中的压力标志物,但它没有改变关键蛋白质合成或生长因素.
科学领域:
- 生物化学 生物化学
- 整形外科 整形外科 整形外科
- 内分泌学 在内分泌学.
背景情况:
- 椎间盘 (IVD) 退化是2型糖尿病 (T2DM) 患者腰部疼痛的重要原因.
- 在T2DM中IVD退化的精确机制和维生素K2的作用尚未完全理解.
研究的目的:
- 调查T2DM和维生素K2补充剂对大鼠模型中IVD退行变化的影响.
- 分析IVD中细胞和分子变化的影响.
主要方法:
- 使用的雄性Zucker糖尿病脂肪 (ZDF) 鼠,非糖尿病和糖尿病,有和没有维生素K2补充.
- 评估了大腿骨的长度,脊椎部区域,IVD组织病理学,纤维肌细胞蛋白质合成 (IL-10,IL-24) 和基因表达 (αSMA,aggrecan,原蛋白类型2,Socs3,Hmox1).
主要成果:
- 糖尿病大鼠表现出基因病理性IVD退化和腿部长度/部区域的缩小,不论维生素K2.
- 维生素K2补充剂在糖尿病IVD细胞中增加了α光滑肌肉动蛋白 (αSMA) 表达,并增强了细胞因子信号传导3 (Socs3) 抑制剂和血氧酶1 (Hmox1) 的基因表达.
- 在糖尿病IVD患者中,Aggrecan基因表达低,而2型原蛋白基因表达高,由于维生素K2没有显著变化. 干白素 (IL) - 10和IL - 24的合成保持不变.
结论:
- 在老鼠中,T2DM诱导了显著的IVD退化.
- 维生素K2在糖尿病IVD细胞中影响特定的与压力相关的基因表达和αSMA,但不会逆转退行性变化或影响IL-10/IL-24合成.
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