在多极端友红色微藻10D中工程化基托卡罗类生物合成Cyanidioschyzon merolae
Mark Seger1, Fakhriyya Mammadova1, Melany Villegas-Valencia2
1Arizona Center for Algae Technology and Innovation, Arizona State University, 7418 Innovation Way South, Mesa, AZ, 85212, United States.
Metabolic engineering communications
|July 14, 2023
概括
工程红色微藻,Cyanidioschyzon merolae,现在可以产生有价值的类胡卜素,如色素. 这一突破使得生物技术应用的 phycocyanin 和 ketocarotenoids 的同时生产成为可能.
科学领域:
- 生物技术是生物技术.
- 微藻工程 微藻工程
- 代谢工程是代谢工程.
背景情况:
- 多极性红色微藻 (Cyanidiophyceae) 提供了生物技术优势,因为它们对极端条件的弹性.
- 化藻10D (Cyanidioschyzon merolae 10D) 是一种缺乏细胞壁的红藻,它在遗传学上可用于核转基因集成.
- C. merolae 具有有限的胡卜素特征,这为代谢工程提供了机会.
研究的目的:
- 通过表达来自绿藻的异质酶,对C. merolae 10D进行基托胡卜素生产的工程设计.
- 通过代谢工程来研究C. merolae通过代谢工程来积累色素的能力.
- 评估色素积累对其他色素特征和生物质生产的影响.
主要方法:
- 构建了一个合成转基因表达系统,使用Clamydomonas reinhardtii (Cr) 酶的编码序列.
- 化原生C. merolae转录,转化和叶绿体向Cr酶的信号.
- 将转基因集成到C. merolae 10D的中性基因组部位中,使用氨基醇耐药性进行选择.
主要成果:
- 表达Cr-BKT导致康塔桑和阿多尼鲁宾的积累.
- 同时表达的Cr-BKT和Cr-CHYB导致阿斯塔山丁作为主要的胡卜素.
- 类胡卜素积累并没有减少总胡卜素,但减少了叶绿素a;植物水平保持稳定. 在持续照明下,生物质生产得到了增强.
结论:
- 这项研究证明了在红色真核微藻中首次成功地进行了胡卜素代谢工程.
- 经过工程设计的C. merolae 10D可以同时产生植物素和有价值的类胡卜素.
- 这为在双产品生物技术应用中使用C. merolae 10D开辟了可能性.
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