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装有微囊的老化纳米塑料在人类肝细胞中引起了代谢转变

Yixin He¹, Ai Shen², Muhammad Salam¹

¹Key Laboratory of Eco-Environment of Three Gorges Region, Ministry of Education, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing 400044, China.

Environmental science & technology

|July 14, 2023

概括

老龄化增加了纳米塑料的微囊素吸附,增强了它们对人类肝细胞的毒性. 携带微囊的老化纳米塑料破坏细胞过程,对人类健康构成风险.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
纳米技术纳米技术

背景情况:

纳米塑料 (NP) 是新兴的环境污染物.
污染物加载的核电站对人类健康的风险存在有限的研究.
微囊 (MCs) 是在水生环境中发现的强有力的毒素.

研究的目的:

评估原始和老化的聚乙烯纳米塑料 (PSNPs) 吸附微囊 (MCs).
在人类肝细胞母细胞瘤 (HepG2) 细胞中研究老化和MC载荷PSNP的分子毒性.
阐明NP诱导的细胞损伤背后的机制.

主要方法:

制备各种度 (1-19μg/mg) 的含有MC的老化PSNP.
暴露HepG2细胞与原始,老化和MCs载荷的老化PSNP.
评估细胞活力,氧化损伤,内质网膜应激和蛋白质合成.
使用奥米克方法分析分子变化.

主要成果:

随着年龄的增长,PSNP的MC-LR吸附量显著增加 (2.64倍).
在HepG2细胞中观察到PSNP的积累.
负载MC-LR的老年PSNP的细胞毒性与MC-LR负载正相关.
衰老的PSNP诱导了内质网膜应激和减少了蛋白质合成.
加载MC-LR的老化PSNP通过代谢障碍,氧化损伤,ER压力和向蛋白降低调节加剧了细胞毒性.

结论:

陈旧的PSNP增强了肝细胞的MC-LR吸收,增加了毒性.
装有微囊的纳米塑料破坏了关键的细胞功能.
这项研究提供了关于纳米塑料带来的人类健康风险的见解.

关键词:

在HepG2细胞中.微囊是一种微囊.纳米塑料是一种纳米塑料.蛋白质组分析蛋白质组分析毒性的毒性毒性的毒性

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