从Aspergillus niger SA1发酵过程和数学建模中生物活性剂生产的统计优化
Mansour A Al-Hazmi1, Tarek A A Moussa2, Nuha M Alhazmi3
1Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80200, Jeddah 21589, Saudi Arabia.
Journal of microbiology and biotechnology
|July 14, 2023
概括
来自沙特阿拉伯的土壤真菌被选为生物表面活性剂的生产. 使用Aspergillus niger SA1的优化条件显著增加了生物表面活性剂的产量,证明了生物修复应用的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 石油污染给环境带来了挑战.
- 生物表面活性剂为石油泄漏整治提供了一个可持续的解决方案.
- 土壤真菌是新型生物表面活性化合物的有希望的来源.
研究的目的:
- 从沙特阿拉伯污染的石油土壤中分离和识别产生生物表面活性剂的真菌.
- 为了优化生物表面活性剂的生产,使用最强大的真菌分离物.
- 评估生物表面活性剂在降低表面张力和乳化油方面的有效性.
主要方法:
- 从受污染的土壤中分离真菌,并通过ITS测序进行识别.
- 使用表面张力,滴落崩和乳化试验来选生物表面活性剂的活性.
- 通过使用Fit Group模型和废油作为基质,优化*Aspergillus niger* SA1的生物表面活性剂生产.
主要成果:
- 在44种真菌分离物中,有39种表现出生物表面活性.
- *Aspergillus niger* SA1显示出高生物表面活性剂的产生 (70%的乳化指数).
- 优化条件提高了生物表面活性剂的产量1.22倍,达到8.02g/l,降低了表面张力和改善了油位移.
结论:
- *Aspergillus niger* SA1是一种高效的生物表面活性剂生产者.
- 优化显著改善了生物表面活性剂的产量和活性.
- 这些发现凸显了真菌生物表面活性剂在环境应用中的潜力,特别是在受油污染的场所.
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