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类似转化因子针对不同的mRNA,以差异调节酵母菌对氧化应激的耐受性
Joanne Cunningham1, Aristeidis P Sfakianos1, Paraskevi Kritsiligkou1
1Faculty of Biology, Medicine and Health, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
Nucleic acids research
|July 14, 2023
概括
翻译启动因子4G (eIF4G) 异型具有不同的作用. eIF4G1,而不是eIF4G2,对于酵母和人类细胞在氧化应激过程中翻译mRNA至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 翻译启动因子4G (eIF4G) 对于真核细胞mRNA翻译至关重要.
- 存在多种eIF4G异型,但它们的特定功能,特别是mRNA特异性翻译调节,尚不清楚.
- 酵母菌Saccharomyces cerevisiae有两个eIF4G异型,eIF4G1和eIF4G2,通常被认为是功能冗余的.
研究的目的:
- 研究eIF4G1和eIF4G2在酵母转化中的特定作用.
- 为了确定eIF4G异型是否以特定于mRNA的方式调节翻译,特别是在压力条件下.
- 为了探索eIF4G异形函数的进化保存.
主要方法:
- 使用的同源酵母菌株表达eIF4G1或eIF4G2作为唯一的异型.
- 采用定量蛋白质组学来分析氧化应激下的蛋白质组变化.
- 研究了蛋白质与蛋白质的相互作用,特别是eIF4G与Slf1 LARP蛋白结合.
主要成果:
- eIF4G1,但不是eIF4G2,特别需要用于酵母体对氧化应激的转化反应.
- eIF4G1在氧化应激过程中结合mRNA盖并积极翻译核糖体,促进应激特异性蛋白质组变化.
- eIF4G1与Slf1 LARP蛋白相互作用,调解氧化应激反应,在人类细胞中观察到类似的作用.
结论:
- 酵母菌的eIF4G异型,eIF4G1和eIF4G2,具有不同的,非冗余的功能.
- eIF4G1在氧化应激下维持翻译起着至关重要的作用,这是人类细胞中保存的功能.
- eIF4G异形的融合进化为在压力条件下的翻译提供了优势.
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