相关实验视频

Updated: Jul 23, 2025

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

通过miR-122-5p/miR-204-5p/HMGB1轴,Circ_LRP6可以促进骨肉瘤的进展

Guanghui Wang¹, Xiyuan Wei², Shan Gao¹

¹Department of Orthopedic Surgery, Zhumadian Central Hospital, Zhumadian, Henan, China.

Environmental toxicology

|July 14, 2023

概括

循环RNA LRP6 (circ_LRP6) 通过调节miR-122-5p/miR-204-5p/HMGB1轴来促进骨髓瘤的进展. 这项研究确定circ_LRP6作为骨质肉瘤的潜在生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

循环RNA LRP6 (circ_LRP6) 与各种癌症有关.
在骨髓瘤 (OS) 中circ_LRP6的特定作用和机制在很大程度上仍未被阐明.

研究的目的:

研究circ_LRP6在骨髓瘤发育中的功能和潜在的分子机制.
为了确定circ_LRP6是否作为骨髓瘤的潜在诊断生物标志物.

主要方法:

定量实时PCR (qRT-PCR) 用于测量circ_LRP6,miR-122-5p,miR-204-5p和HMGB1.1的表达水平.
细胞计数套件-8 (CCK-8) 和Transwell测定用于评估细胞增殖,迁移和入侵.
生物信息学分析和双露西法酶记者试验验证实了分子相互作用.
在体内研究使用裸体小鼠皮下瘤形成模型.

主要成果:

与正常组织相比,骨髓瘤组织表现出circ_LRP6和HMGB1的显著更高的表达,以及miR-122-5p和miR-204-5p的表达较低.
Knockdown of circ_LRP6显著抑制了骨髓瘤细胞的增殖,迁移和入侵 in vitro 和瘤生长 in vivo.
生物信息学和实验验证证证了circ_LRP6的目标是miR-122-5p和miR-204-5p,这反过来又针对HMGB1.
恢复miR-122-5p/miR-204-5p或HMGB1部分扭转了circ_LRP6淘汰的影响.

结论:

Circ_LRP6通过miR-122-5p/miR-204-5p/HMGB1信号通路促进骨肉瘤的进展.
Circ_LRP6 作为骨髓瘤的潜在分子生物标志物.

关键词:

这就是HMGB1的特点.环_LRP6 的情况.在 miR-122-5p 中使用.在 miR-204-5p 中.骨质肉瘤是骨质肉瘤的一种疾病.它们的扩散和扩散.

更多相关视频

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Published on: June 13, 2018

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...