相关实验视频
Updated: Jul 23, 2025

11:29
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
10.9K
以METTL3为媒介的m6A修改pri-miR-148a-3p通过调节TXNIP影响前列腺癌的进展
Guoqiang Li1, Junwen Liu2, Yinhuai Wang1
1Department of Urology, The Second Xiangya Hospital, Central South University, Changsha, China.
Environmental toxicology
|July 14, 2023
概括
甲基转移酶样3 (METTL3) 通过通过m6A修饰对miR-148a-3p进行上调,从而促进前列腺癌 (PCa),从而使TXNIP沉默. 干扰METTL3可以抑制PCa的进展和瘤的生长.
科学领域:
- 分子瘤学分子瘤学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组RNA的修改 基因组RNA的修改
背景情况:
- 前列腺癌 (PCa) 是男性的重大全球健康问题.
- 甲基转移酶类3 (METTL3) 在PCa病变发生中的作用需要阐明.
- 越来越多的N6-甲基氨酸 (m6A) 修饰因其在癌症中的作用而得到认可.
研究的目的:
- 调查METTL3影响PCa发展的机制.
- 确定PCa中METTL3,miR-148a-3p和TXNIP之间的监管关系.
- 探索针对METTL3/miR-148a-3p/TXNIP轴在PCa治疗中的潜力.
主要方法:
- 定量实时PCR (RT-qPCR) 和西式涂抹评估了基因和蛋白质的表达.
- Me-RIP测定量化了 pri-miR-148a-3p 的 m6A 修饰水平.
- 在体外 (基于细胞的测定) 和体内 (异种移植小鼠模型) 实验中评估了PCa细胞行为和瘤生长.
主要成果:
- METTL3干扰抑制了PCa细胞的增殖,迁移和入侵,同时促进了细胞亡.
- 通过m6A修改 pri-miR-148a-3p,METTL3 调高了 miR-148a-3p 的表达.
- miR-148a-3p过度表达逆转了METTL3沉默的抑制作用;miR-148a-3p沉默了TXNIP,促进了PCa细胞的生长.
结论:
- METTL3通过m6A修饰增强miR-148a-3p来促进PCa的进展,从而导致TXNIP下调.
- 向METTL3有效地抑制PCa细胞的增殖,迁移,入侵和瘤生长.
- METTL3/miR-148a-3p/TXNIP通路代表了前列腺癌的潜在治疗标.
相关概念视频
MicroRNAs
21.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.4K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Regulation of Expression at Multiple Steps
945
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
945
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Covalently Linked Protein Regulators
6.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.9K
PI3K/mTOR/AKT Signaling Pathway
3.7K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.7K

