构建基于宽频抗体的高度选择性丰富,电离和检测平台
Jiawei Liu1,2,3, Fengming Chen2,3, Yang Song2,3
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Analytical chemistry
|July 14, 2023
概括
这项研究引入了一种新的涂层刀片喷雾质谱平台,用于快速,选择性地检测食品中的诺基诺药物. 该方法使用抗体功能化石墨烯氧化物增强灵敏度,为食品安全分析提供了强大的工具.
科学领域:
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 环境质谱 (AMS) 能够直接分析食品,但在复杂矩阵中的微量分析物方面存在局限性.
- 目前的方法往往需要大量的样品预处理,这阻碍了快速查.
研究的目的:
- 开发一个快速,现场,高吞吐量和选择性平台,用于在食品样本中对类类药物 (FQs) 的多余量分析.
- 为了提高环境质谱的灵敏度,用于微量分析物的检测.
主要方法:
- 开发了一个涂层不钢板喷雾质谱平台.
- 石墨烯氧化物涂层刀片喷雾剂 (CBS) -MS通过链条维丁-生物素相互作用与宽谱抗FQ抗体功能化.
- 使用MS/MS光谱进行了分析物检测.
主要成果:
- 该平台表现出高负载能力的链状维丁和抗体.
- 在各种矩阵中,灵敏度与高性能液态染色学-质谱学 (HPLC-MS/MS) 相当.
- 实现了快速分析,缩短缩时间 (10分钟) 和检测时间 (<1分钟).
- 获得了可接受的恢复 (81.94-102.08%) 和同时多分析检测.
结论:
- 开发的CBS-MS平台提供了一种强大的,便携式工具,用于快速实验室分析和对食品中的FQ进行现场查.
- 这种方法在食品安全监测的传统技术上取得了显著的进步.
更多相关视频
09:25Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
Published on: February 16, 2017
12.3K
00:09Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Published on: August 22, 2019
10.8K
相关概念视频
01:20Immunoprecipitation
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:03Affinity Chromatography
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...