乳腺癌中的S100A8基因拷贝数和蛋白质表达:与增殖,病原学等级和分子亚型的关联
Mathieu Le Boulvais Børkja1, Miriam S Giambelluca2,3, Borgny Ytterhus4
1Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, Trondheim, Norway. mlborkja@stud.ntnu.no.
乳腺癌细胞中的S100A8蛋白表达,而不是基因放大,与更差的预后有关. 这种蛋白质可以识别出侵略性的非光线瘤的一个子组.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- S100A8基因放大发生在10-30%的乳腺癌中,与更差的预后有关.
- 过度表达S100A8蛋白质在乳腺癌和透髓状细胞的相似比例中被发现,也与糟糕的结果有关.
研究的目的:
- 研究S100A8拷贝数 (CN) 变化与乳腺癌中S100A8蛋白表达之间的关系.
- 为了确定S100A8 CN和乳腺癌中的蛋白质表达的预后意义.
主要方法:
- 在475种原发性乳腺癌中使用光 in situ 杂交分析了S100A8 CN.
- 在498种原发性乳腺癌中通过免疫组织化学评估S100A8蛋白表达.
- 与临床病理特征和患者预后相关的S100A8变化.
主要成果:
- 在20%的瘤细胞中增加了S100A8 CN;增加了15%的蛋白质表达.
- 增加的S100A8 CN和蛋白质表达都与高Ki67,高线粒细胞数和高基因病理学等级相关.
- 只有S100A8蛋白在癌细胞中的表达与更差的预后有显著的关联.
结论:
- 在乳腺癌中,S100A8基因放大与S100A8蛋白表达无关.
- 瘤上皮细胞中的S100A8蛋白表达确定了一个主要非光线瘤的子组,预后明显更差.
- 单独使用S100A8并不足以识别与更差预后相关的透免疫细胞.
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