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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Overview of Exosomes01:36

Overview of Exosomes

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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相关实验视频

Updated: Jul 23, 2025

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
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Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

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从体液样本进行外体RNA测序.

Xiaoshuang Li1, Xingyu Liu1, Xiaoke Wei1

  • 1Genesis, Beijing, China.

Methods in molecular biology (Clifton, N.J.)
|July 14, 2023
PubMed
概括

外体,细胞释放的微小囊泡,携带RNA进行细胞间通信. 分析外体RNA配置文件有助于检测癌症生物标志物和了解癌症进展.

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 癌症研究 癌症研究

背景情况:

  • 外体是细胞外囊泡,参与细胞间通信.
  • 来自癌细胞的外基因组有助于瘤的产生,侵袭和转移.
  • 包括RNA在内的外体含量可以用作生物标志物.

研究的目的:

  • 详细说明了外体RNA测序的实验程序.
  • 概述一种生物信息学管道,用于分析外体RNA数据.
  • 为了促进从外体RNA中识别与癌症相关的生物标志物.

主要方法:

  • 从生物流体中分离出外体.
  • 外体RNA的高通量测序. 外体RNA的高通量测序.
  • 对RNA测序数据的生物信息分析.

主要成果:

  • 建立了对外体RNA分析的全面工作流程.
  • 确定了潜在的与癌症相关的RNA特征.
  • 证明了外体RNA测序在癌症研究中的实用性.

结论:

关键词:
早期诊断 早期诊断 早期诊断外体RNA生物标志物外基因组RNA测序异构体隔离异构体的隔离液体活检液体活检

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  • 外基因组RNA测序是癌症生物标志物发现的强大工具.
  • 描述的方法和管道允许对外体RNA进行可靠的分析.
  • 这种方法促进了对癌症中外体介导细胞间通信的理解.