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Updated: Jul 23, 2025

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

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动态丰富用于评估蛋白质网络 (DEEPN):一个高通量酵母双混合 (Y2H) 协议来评估网络

Ali Zeeshan Fakhar1, Jinbao Liu1, Karolina M Pajerowska-Mukhtar2

  • 1Department of Biology at University of Alabama, Birmingham, AL, USA.

Methods in molecular biology (Clifton, N.J.)
|July 14, 2023
PubMed
概括

研究人员开发了DEEPN (用于评估蛋白质网络的动态丰富) 以改进蛋白质-蛋白质相互作用 (PPI) 映射. 这种方法使用下一代测序和计算分析来识别单个酵母-两个混合体实验中的多个相互作用.

关键词:
在 DEEPN 里面.高通量的高通量.照明的测序 Illumina 测序这是一个PPI PPI.系统生物学 系统生物学Y2H 是一个很好的时间.

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相关概念视频

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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