人类核蛋白SURF6/RRP14参与了前rRNA处理的早期步骤
Anastasiia Moraleva1, Alexander Deryabin1, Maria Kordyukova2
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russian Federation.
PloS one
|July 14, 2023
概括
人类的SURF6蛋白对核糖体生物生成至关重要,影响rRNA成熟和细胞周期进展. 它的淘汰改变了细胞循环阶段,独立于p53,这表明它在细胞过程中扮演了多个角色.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 核糖体生物生成涉及复杂的核糖体前RNA (pre-rRNA) 处理和子单元组装.
- 酵母Rrp14和小鼠Surf6等蛋白质参与了早期的rRNA前处理.
- 人类SURF6同类在哺乳动物核糖体生物发生中的作用需要澄清,因为复杂性.
研究的目的:
- 在前rRNA处理的早期阶段阐明人类SURF6的功能.
- 研究SURF6调制对人类细胞系rRNA成熟的影响.
- 确定SURF6对细胞周期进展及其潜在的p53依赖性的影响.
主要方法:
- 在HeLa和HCT116细胞系中利用了人类SURF6的淘汰和过度表达.
- 分析了rRNA前体的丰富性,以评估SURF6在rRNA成熟中的作用.
- 在p53-充足和p53-缺乏的HCT116细胞中进行细胞周期分布分析.
主要成果:
- 人类SURF6对于小和大核糖体亚单元RNA的成熟至关重要.
- SURF6调制改变了细胞周期相位分布,而不会导致HeLa细胞的细胞死亡.
- SURF6敲击诱导细胞周期变化 (G0/G1延长,G2/M缩短) 独立于p53状态.
结论:
- 人类的SURF6在rRNA生物发生和成熟中起着至关重要的作用.
- SURF6以p53独立的方式影响细胞周期进展,表明更广泛的细胞功能.
- SURF6的淘汰可能会诱导rRNA生物发生路径的转变,可能与p53路径联系起来.
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