Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
Types of RNA01:20

Types of RNA

5.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.9K
Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
RNA Interference01:23

RNA Interference

26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Triazole-Sulfonamide Hybrids as Carbonic Anhydrase Inhibitors: Emerging Chemistry and Bioactivity Insights From 2020 to 2025.

Archiv der Pharmazie·2026
Same author

Dual-Function Lipid-Based Nanovector Strategy for Glioblastoma Immunotherapy: STING Activation and M1 Microglia Polarization.

Drug development research·2026
Same author

Correction: Fahmy et al. Palladium Nanoparticles Fabricated by Green Chemistry: Promising Chemotherapeutic, Antioxidant and Antimicrobial Agents. <i>Materials</i> 2020, <i>13</i>, 3661.

Materials (Basel, Switzerland)·2026
Same author

Correction: Fahmy et al. PEGylated Chitosan Nanoparticles Encapsulating Ascorbic Acid and Oxaliplatin Exhibit Dramatic Apoptotic Effects against Breast Cancer Cells. <i>Pharmaceutics</i> 2022, <i>14</i>, 407.

Pharmaceutics·2026
Same author

Cytochrome P450-derived arachidonic acid metabolites as emerging therapeutic targets in menopause-associated cardiac hypertrophy.

Drug metabolism reviews·2026
Same author

<i>HOTTIP</i>-Variants in Liver Cancer Metastasis Prognosis: A Clinical Study with Bioinformatics and siRNAs Targeting HOTTIP-WDR5 via Molecular Docking, a Step-Toward ncRNA Precision.

International journal of molecular sciences·2026
Same journal

Mt1-Ca<sup>2+</sup>-Mitochondrial Metabolic Axis Maintains Temporomandibular Joint Condylar Cartilage Homeostasis Under Low Oxygen and Hypoxic Condition.

Journal of cellular physiology·2026
Same journal

Correction to "IRE1α/NOX4 Signaling Pathway Mediates Ros-Dependent Activation of Hepatic Stellate Cells in NaAsO<sub>2</sub>-Induced Liver Fibrosis".

Journal of cellular physiology·2026
Same journal

Lipocalin-2 Restores Mitochondrial and Antioxidant Adaptation in a Strain-Specific Manner During LPS Induced Sepsis.

Journal of cellular physiology·2026
Same journal

Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens.

Journal of cellular physiology·2026
Same journal

Corrigendum to "A Probiotic Combination of Limosilactobacillus reuteri and Clostridium butyricum Ameliorates 5-Fluorouracil-Induced Intestinal Mucositis in Rats by Strengthening Barrier Integrity and Modulating Immuno-Microbial Homeostasis".

Journal of cellular physiology·2026
Same journal

Sexually Dimorphic Regulation of MiR-29a/c-3p in Human Endothelial Cells: Cell Functions and Transcriptome.

Journal of cellular physiology·2026
查看所有相关文章

相关实验视频

Updated: Jul 23, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

465

从非编码RNA的角度来解码肝癌发生.

Mostafa K Abd El-Aziz1,2, Alyaa Dawoud3, Caroline J Kiriacos3

  • 1Biochemistry Department, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut, Egypt.

Journal of cellular physiology
|July 14, 2023
PubMed
概括
此摘要是机器生成的。

长非编码RNAs (lncRNAs) 和圆形RNAs (circRNAs) 越来越多地与肝细胞癌 (HCC) 的发展有关. 了解它们的作用为HCC诊断,预后和药物开发提供了新的途径.

关键词:
链接RNAss 的时间.环绕RNAss 的时间.肝细胞癌 (HCC) 是一种肝细胞癌.在 lncRNAs 中.微型RNAs (miRNAs) 是一种微型RNA.

更多相关视频

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

6.9K
Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

8.1K

相关实验视频

Last Updated: Jul 23, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

465
Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

6.9K
Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

8.1K

科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 肝细胞癌 (HCC) 是一种主要的全球性癌症,肝脏瘤发生机制尚不清楚.
  • 虽然研究了microRNAs,但长非编码RNAs (lncRNAs) 和圆形RNAs (circRNAs) 在HCC中的作用仍未得到研究.
  • lncRNAs调节各种细胞功能,它们的失调与HCC的各种病因有关.

研究的目的:

  • 审查最近在HCC病理生理学中关于lncRNA和circRNA失调的发现.
  • 探索lncRNAs和circRNAs作为HCC新型诊断和预后生物标志物的潜力.
  • 讨论未来的趋势和针对HCC中的lncRNAs和circRNAs的治疗策略.

主要方法:

  • 关于最近有关HCC的lncRNA和circRNA研究突破的文献综述.
  • 对lncRNAs和circRNAs在HCC进展中的病理作用的分析.
  • 基于lncRNA和circRNA目标的潜在药物开发策略的讨论.

主要成果:

  • lncRNAs和circRNAs在HCC病理生理学中起着重要作用.
  • 失调的lncRNAs和circRNAs显示出作为诊断和预后生物标志物的前景.
  • 这些非编码RNAs影响HCC的增殖,迁移和上皮细胞到介质细胞的过渡.

结论:

  • lncRNAs和circRNAs代表了理解HCC的关键研究领域.
  • 准lncRNAs和circRNAs有可能用于新的HCC诊断,预后和治疗应用.
  • 对lncRNAs和circRNAs的进一步研究可以推动有效的HCC药物开发.