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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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相关实验视频

Updated: Jul 23, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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基于光学连贯断层扫描的设计,用于实时运动校正扫描显微镜.

Stephen S Tucker, John T Giblin, Kivilcim Kiliç

    Optics letters
    |July 14, 2023
    PubMed
    概括

    这项研究引入了用于双光子光显微镜的运动跟踪模块,纠正样本运动引起的扭曲. 这项创新提高了动态生物研究的成像质量.

    科学领域:

    • 生物医学工程 生物医学工程
    • 光学成像技术的成像
    • 显微镜的使用方法

    背景情况:

    • 双光子光显微镜对于研究活细胞和组织动态至关重要.
    • 在成像过程中,样品的运动引入了显著的扭曲,限制了数据的准确性.
    • 现有的方法可能需要对比剂或复杂的设置.

    研究的目的:

    • 开发一个实时运动跟踪和校正模块,用于激光扫描双光子显微镜.
    • 整合光学连贯断层扫描 (OCT) 进行运动补偿,无需额外的对比剂.
    • 为了提高动态生物成像在大量运动的情况下的保真度.

    主要方法:

    • 设计了一种基于光谱域光连贯断层扫描 (SD-OCT) 的运动跟踪模块.
    • 集成模块与激光扫描二光子显微镜,使用单个二合体镜.
    • 实现了侧向运动补偿的实时校正反.

    主要成果:

    • 该系统成功地跟踪了5Hz的侧向位移,长度高达10μm.
    • 实现了低于14ms的反延迟,用于实时校正.
    • 在不需要额外的对比剂的情况下证明了集成.

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    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
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    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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    结论:

    • 开发的运动跟踪模块在双光子显微镜中有效地纠正运动工件.
    • 该系统为增强的动态成像提供了一个非侵入性的,可适应的解决方案.
    • 未来的工作包括3D校正和延迟减少,以获得更广泛的适用性.