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相关概念视频

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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相关实验视频

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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
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一个基于微光探测器阵列和动态零校正算法的96井板紫外线光仪.

Chuanliang Wang1, Xuhui Geng2, Yan Gao2

  • 1Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Key Laboratory of Deep-sea Composition Detection Technology of Liaoning Province, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Talanta
|July 14, 2023
PubMed
概括

一种新的96井板紫外线光仪使用8通道检测器阵列进行快速分析. 它的动态零校正算法显著提高了高通量选的准确性和检测极限.

关键词:
96-井板紫外线光计 井板紫外线光计 井板紫外线光计动态零校正算法 动态零校正算法微光探测器阵列是一个微光探测器阵列.

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Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

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科学领域:

  • 分析化学 分析化学
  • 仪器科学 仪器科学

背景情况:

  • 准确和高效的光检测对于各种科学领域的高通量选至关重要.
  • 现有的度仪面临着背景光和测量精度的挑战.

研究的目的:

  • 为提高检测效率和准确性,开发和评估一款全新的96井板紫外线光仪.
  • 为了解决由背景光变化引起的测量精度限制.

主要方法:

  • 开发使用紫外线发光二极管 (LED) 和光二极管 (PD) 的8通道探测器阵列.
  • 实施光线跟踪用于光路设计,以防止井交叉通话.
  • 引入动态零校正算法以减轻背景光效应.
  • 在8个频道同时扫描和检测,以节省时间.

主要成果:

  • 实现了大约80秒的扫描时间,用于96井板.
  • 对于1μg/L标准,已证明测量可重复性 (RSD) 为2.25%.
  • 与固定零校正相比,显著改善了检测极限 (LOD),可重复性和减少了平均相对误差.

结论:

  • 开发的紫外线度仪提供了强大的性能和高通量分析的潜力.
  • 动态零校正算法提高了测量准确性和可靠性.
  • 这种仪器对食品安全和生命科学领域的应用具有前景,需要快速检测.