比较理论和实际的生物质产量要求重新审视电活性微生物的热力学生长模型
Benjamin Korth1, João Pereira2, Tom Sleutels3
1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research GmbH - UFZ, Permoserstr. 15, Leipzig 04318, Germany.
Water research
|July 14, 2023
概括
电活性微生物 (EAM) 显示能量收获受限,实验生物质产量与模型预测不同. 这表明没有考虑的能量消耗和需要调整的增长模型,以更好地了解微生物电化学技术 (MET).
科学领域:
- 微生物学 微生物学
- 生物电化学 生物电化学
- 生物技术是生物技术.
背景情况:
- 电活性微生物 (EAM) 的研究主要探讨了微生物电化学技术 (MET) 中的细胞外电子转移机制和应用.
- 对EAM增长和活动中的能量转换的热力学理解仍未得到充分探索.
- 现有的EAM增长模型可能无法准确预测生物质产量.
研究的目的:
- 为了研究EAM中假设的受限能量收获.
- 为了确定不同阳极电位和酸盐度下的酸盐养生物膜的生物质产量.
- 将实验生物质产量与精细热力学模型的预测进行比较.
主要方法:
- 使用光学相干断层扫描监测酸盐养生物膜的生长.
- 在三种不同的阳极电位和四种酸盐度进行实验.
- 模拟实验条件使用EAM精细的热力学模型.
主要成果:
- 在生物质产量和阳极潜力或酸盐度之间没有明确的相关性.
- 基于乙酸消费的实验生物质产量 (YX/ac = 37 ± 9 mgCOD生物质 gCODac-1) 超过了模型估计.
- 基于触媒能量收获的模拟生物质产量 (YX/cat = 25.9 ± 6.8 mgCOD生物质 kJ-1) 比实验数据高,支持受限制的能量收获.
结论:
- 实验发现表明当前EAM增长模型的局限性.
- 在EAM中支持有限的能量收获,不考虑的能量吸收器的潜在贡献,如微生物电化学Peltier热.
- 需要调整的增长模型,包括佩尔蒂埃热等因素,以更好地理解和建模EAM能量代谢和MET可行性.
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