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在diplonemid鞭毛体中的线粒体脱酶复合体的子单元组成

Kristína Záhonová¹, Matus Valach², Pragya Tripathi³

¹Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic; Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic; Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic; Division of Infectious Diseases, Department of Medicine, University of Alberta, Edmonton, Canada.

Biochimica et biophysica acta. General subjects

|July 14, 2023

概括

狄普隆尼米斯在他们的酸盐脱酶复合体中使用了古老的AceE蛋白,而不是典型的E1亚单元. 这表明海洋原生动物中这种关键代谢酶有一个独特的进化途径.

科学领域:

生物化学生化学
分子生物学分子生物学
亲生组织学亲生组织学亲生组织学

背景情况:

核生物的酸盐代谢依赖于线粒体的酸盐脱酶 (PDH) 复合体.
狄普隆尼米,海洋原生体丰富,缺乏传统的PDH子单元.
在一些euglenozoans (包括diplonemids) 中,一个archaeal AceE蛋白取代了E1亚单元.

研究的目的:

在模型Diplonemid Paradiplonema papillatum中研究PDH复合物的组成和功能.
确定ACEE蛋白在diplonemid pyruvate代谢中的作用.
阐明了diplonemids中PDH复合物的进化适应.

主要方法:

进行比较的转录组分析.
线粒体酶活性测定 (pyruvate和2-oxoglutarate脱酶活性).
使用质谱学进行蛋白质组学分析.

主要成果:

在Paradiplonema papillatum的线粒体中表现出pyruvate和2-oxoglutarate脱酶的活性.
古代的ACEE蛋白在P. papillatum线粒体中非常丰富,与BCKDH E1亚单元相似.
证实AceE是diplonemids中PDH复合物的功能成分.

结论:

狄普隆尼米类动物具有改性PDH复合体,其中古老的AceE蛋白替代了真核生物的E1亚单元.
这种适应可能导致了传统E2和E3子单位的丧失,AceE可能与BCKDH和/或OGDH的子单位合作.
这项研究揭示了一种独特的进化策略,用于一大群海洋原生体的中央碳代谢.

关键词:

脱水酶复合体脱水酶复合体双斑 (Diplonema papillatum) 是一种发生在皮肤上的疾病.双半体动物 (Diplonemids) 是一种双半体动物.进化进化进化进化进化进化进化线粒体中的线粒体.抗议主义者抗议主义者

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