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对大肠杆菌进行多维工程,以高效合成L-三

Mi Tang¹, Xuewei Pan¹, Tianjin Yang¹

¹Key Laboratory of Industrial Biotechnology of the Ministry of Education, Laboratory of Applied Microorganisms and Metabolic Engineering, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Yixing Institute of Food and Biotechnology Co., Ltd, Yixing 214200, China.

Bioresource technology

|July 14, 2023

概括

这项研究开发了一种高通量选平台,用于设计微生物细胞工厂,以提高L-托 (L-trp) 产量,达到42.5g/L的产量.

科学领域:

代谢工程是代谢工程.
合成生物学合成生物学
生物技术是生物技术.

背景情况:

微生物生产L-三 (L-trp) 面临的挑战是代谢流量和低产量.
开发高效的微生物细胞工厂需要先进的工程策略.

研究的目的:

使用基于 рибо开关的高通量选 (HTS) 平台构建一个强大的L-trp产生微生物底盘.
通过多维代谢调节优化L-trp生物合成.

主要方法:

为L-trp生产建立了一个基于Riboswitch的HTS平台.
通过促进体和N-终端编码序列 (NCS) 修改工程基因表达.
微调的代谢模块 (降解,运输,副产品合成) 和识别的调节因子 (YihL).
实施了分支路径的动态调节,并改善了辅因子供应.

主要成果:

从工程菌株Trp30获得42.5g/L L-trp和0.178g/g葡萄糖的高产量.
在48小时内在5L生物反应器中成功培养,没有额外的补充剂.
验证了将HTS与多维调节相结合的有效性.

结论:

开发的HTS平台和多维调节策略为创建高效的细胞工厂提供了有希望的方法.
这项工作显著提高了微生物L-trp生产能力.
该方法在各种目标产品的工程电池工厂中广泛适用.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.高通量选的高通量选 L-三甲是一种L-三甲.多维工程是多维的工程.在Riboswitch中使用.

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