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通过真空透加速冷保护剂的递送.

Ryan J Forcier1, Robert T Heussner1, Lauren Newsom2

  • 1School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR, USA.

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|July 14, 2023
PubMed
概括
此摘要是机器生成的。

脊椎体 (VBs) 的冷保存是困难的. 碳化和真空循环可能会略微改善冷保护剂的输送到像VBs这样的大组织中.

关键词:
这是一种冷保护剂.质量转移是指质量转移.机器人 机器人 机器人 机器人组织组织组织组织.在真空中吸尘.脊椎体是一个体.

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科学领域:

  • 生物医学工程 生物医学工程
  • 组织工程是组织工程.
  • 低温生物学 低温生物学

背景情况:

  • 大型生物结构的冷保存,如脊椎体 (VBs),受到大规模运输挑战的限制.
  • 将冷保护剂有效地输送到像VB这样的密集组织中,对于成功的冷保存至关重要.
  • 目前的方法难以在大量组织中实现冷保护剂的均分布.

研究的目的:

  • 调查碳化和真空循环的有效性,以增强冷保护剂透到VBs.
  • 确定二氧化碳气体透后的真空循环是否可以改善大型组织结构中的冷保护剂的输送.
  • 评估这种方法在推进临床和研究应用中VB冷保存方面的潜力.

主要方法:

  • 使用阿加罗斯凝和彩色染料作为模型系统来评估透增强.
  • 应用碳化 (高压二氧化碳气体),然后进行真空循环到模型系统和人体VBs.
  • 使用CT成像可视化气泡并评估VBs中的DMSO度.

主要成果:

  • 在阿加凝模型中,碳化和真空循环增加了14%的染料透率.
  • CT成像证实了VB中存在气泡,真空循环使气泡体积减少了50%以上.
  • 没有通过CT成像检测到VB内DMSO度的统计显著增加.

结论:

  • 碳化和真空循环显示了冷保护剂在像VBs这样的大组织结构中的适度改善的潜力.
  • 需要进一步的研究来优化该技术并确认其对VB冷保存的有效性.
  • 这种方法可以为用于移植或研究目的的大型组织的保存提供好处.