自动化大容量样本准备用于vEM的自动化样本准备
Erin S Stempinski1, Lucas Pagano2, Jessica L Riesterer3
1Multiscale Microscopy Core, Oregon Health & Science University, Portland, OR, United States.
Methods in cell biology
|July 14, 2023
概括
自动化样本处理显著加快了体积电子显微镜 (vEM) 的样本准备. 这种方法提高了重金属染色的可重复性,并减少了危险化学物质的暴露,从而提高了vEM数据质量.
科学领域:
- 生命科学 生命科学
- 显微镜的使用方法
- 生物技术是生物技术.
背景情况:
- 由于技术进步,体积电子显微镜 (vEM) 的使用正在增加.
- 传统的VEM样本准备是耗时的,并且涉及危险的化学物质.
- 优化样品质量和最小化文物对于VEM研究至关重要.
研究的目的:
- 描述一种自动化方法,用于为vEM准备样本.
- 与传统方法相比,评估自动化样品准备的效率和质量.
- 确保与各种vEM技术 (如FIB-SEM,SBF-SEM和AT) 的兼容性.
主要方法:
- 使用mPrepTM ASP-2000TM自动样本处理器进行样本准备.
- 开发了用于vEM技术的协议,包括聚焦离子束扫描电子显微镜 (FIB-SEM),串行块面扫描电子显微镜 (SBF-SEM) 和阵列断层扫描 (AT).
- 将自动化处理结果与用于图像质量分析的广泛使用的常规基板协议进行比较.
主要成果:
- 自动化处理显著减少了样品准备时间.
- 观察到重金属染色的可复制性得到改善.
- 在SEM过程中从回散电子成像中发现的数据质量没有妥协.
- 尽量减少操作员对危险化学品的暴露.
结论:
- 自动化样本处理为VEM样本准备提供了更快,更安全,更可重复的替代方案.
- mPrepTM ASP-2000TM对于准备与多种vEM技术兼容的样品是有效的.
- 这种自动化方法保持了高数据质量,同时提高了工作流的效率.
关键词:
射线断层扫描 (Array tomography) 是一种光谱扫描技术.自动样本处理自动化样本处理电子显微镜的电子显微镜在FIB-SEM中.图像质量 图像质量这就是SBF-SEM.容量-EM-体积可以使用.更多相关视频
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