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相关概念视频

Computed Tomography01:10

Computed Tomography

4.6K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
4.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Positron Emission Tomography01:29

Positron Emission Tomography

4.3K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
4.3K
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

158
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
158
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

28
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
28

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相关实验视频

Updated: Jul 23, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

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一个开始SEM阵列断层扫描的实用指南 - 一个可访问的卷EM技术.

Ian J White1, Jemima J Burden1

  • 1LMCB, University College London, London, United Kingdom.

Methods in cell biology
|July 14, 2023
PubMed
概括

扫描电子显微镜阵列断层扫描 (SEM-AT) 为3D超结构数据采集提供了一种灵活,具有成本效益和非破坏性的方法. 这种技术正在变得越来越容易在电子显微镜设施的日常使用世界各地.

科学领域:

  • * 显微镜的使用
  • * 超结构生物学 超结构生物学
  • * 材料科学 材料科学

背景情况:

  • *体积电子显微镜 (vEM) 涵盖了各种技术,具有明显的优缺点.
  • *扫描电子显微镜阵列断层扫描 (SEM-AT) 是一种灵活,具有成本效益的VEM方法.
  • * SEM-AT需要微切割技能和成像后数据对齐,这给用户带来了挑战.

研究的目的:

  • * 描述SEM-AT的一般原则,优点和缺点.
  • * 为SEM-AT工作流提供一个逐步指南.
  • * 突出SEM-AT成为常规的vEM技术的潜力.

主要方法:

  • * 详细的工作流程从封面片上的块修剪,切割和收集.
  • * 高分辨率3D数据集的对齐.
  • *利用扫描电子显微镜 (SEM) 与反射电子探测器和专门的成像软件.

主要成果:

  • * SEM-AT可使用标准电子显微镜设备进行3D超结构数据采集.
  • *适当的软件可以自动成像数百到数千个部分.
  • *该技术本质上是非破坏性的,提供了显著的优势.
关键词:
射线断层扫描 (Array tomography) 是一种光谱扫描技术.不具有破坏性的非破坏性.这就是SEM-AT.扫描电子显微镜扫描电子显微镜部分对齐 部分对齐序列部分的序列部分.这是一种超微皮切除术 (Ultramicrotomy).卷电子显微镜的体积电子显微镜.

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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

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相关实验视频

Last Updated: Jul 23, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

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结论:

  • * SEM-AT是一种用于普通EM设施的多功能和可访问的vEM技术.
  • * 硬件和软件的进步正在简化这个过程.
  • * SEM-AT有可能成为全球常规的vEM方法.