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Updated: Jul 23, 2025

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加强FIB-SEM样本准备和图像采集的方法

Song Pang1, C Shan Xu2

  • 1Yale School of Medicine, New Haven, CT, United States.

Methods in cell biology
|July 14, 2023
PubMed
概括

增强的聚焦离子束扫描电子显微镜 (FIB-SEM) 改善了细胞成像. 本研究详细介绍了FIB-SEM独特的准备和成像方法,以实现更好的生物发现和提高吞吐量.

关键词:
电子显微镜 (EM) 是一种电子显微镜.增强的FIB-SEM可以使用.投资回报率 (ROI) 准目标样品的准备 样品的准备这是X射线.

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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